ArticleThe Journal of biological chemistry2010
Tryptophan in the pore of the mechanosensitive channel MscS: assessment of pore conformations by fluorescence spectroscopy.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- The physicochemical properties of lipopolysaccharide chemotypes regulate activation of the contact pathway of blood coagulation.The Journal of biological chemistry · 2025Article
- How Functional Lipids Affect the Structure and Gating of Mechanosensitive MscS-like Channels.International journal of molecular sciences · 2022Review
- Mechanosensitive channel gating by delipidation.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Mechanosensitive channel YnaI has lipid-bound extended sensor paddles.Communications biology · 2021Article
- Interaction of the Mechanosensitive Channel, MscS, with the Membrane Bilayer through Lipid Intercalation into Grooves and Pockets.Journal of molecular biology · 2019Article
- Nonpolar residues in the presumptive pore-lining helix of mechanosensitive channel MSL10 influence channel behavior and establish a nonconducting function.Plant direct · 2018Article
- The role of lipids in mechanosensation.Nature structural & molecular biology · 2015Article
- The Structure of YnaI Implies Structural and Mechanistic Conservation in the MscS Family of Mechanosensitive Channels.Structure (London, England : 1993) · 2015Article
- Properties of the Mechanosensitive Channel MscS Pore Revealed by Tryptophan Scanning Mutagenesis.Biochemistry · 2015Article
- Quantification of free cysteines in membrane and soluble proteins using a fluorescent dye and thermal unfolding.Nature protocols · 2013Article
- Salt bridges regulate both dimer formation and monomeric flexibility in HdeB and may have a role in periplasmic chaperone function.Journal of molecular biology · 2012Article
- Bacterial mechanosensitive channels--MscS: evolution's solution to creating sensitivity in function.Annual review of biophysics · 2012Review
- Defining the role of the tension sensor in the mechanosensitive channel of small conductance.Biophysical journal · 2011Article
- Characterization of three novel mechanosensitive channel activities in Escherichia coli.Channels (Austin, Tex.)Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Structural changes in channel proteins give critical insights required for understanding the gating transitions that underpin function. Tryptophan (Trp) is uniquely sensitive to its environment and can be used as a reporter of conformational changes. Here, we have used site-directed Trp insertion within the pore helices of the small mechanosensitive channel protein, MscS, to monitor conformational transitions. We show that Trp can be inserted in place of Leu at the two pore seal positions, Leu(105) and Leu(109), resulting in functional channels. Using Trp(105) as a probe, we demonstrate that the A106V mutation causes a modified conformation in the purified channel protein consistent with a more open state in solution. Moreover, we show that solubilized MscS changes to a more open conformation in the presence of phospholipids or their lysoforms.
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