ArticleCurrent biology : CB2010
The transcription elongation factor NusA is required for stress-induced mutagenesis in Escherichia coli.
Article in Current biology : CB, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 77 citations in OpenAlex.
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- Role of Mfd and GreA in Bacillus subtilis Base Excision Repair-Dependent Stationary-Phase Mutagenesis.Journal of bacteriology · 2020Article
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- DNA polymerase IV primarily operates outside of DNA replication forks in Escherichia coli.PLoS genetics · 2018Article
- Implementation of a loss-of-function system to determine growth and stress-associated mutagenesis in Bacillus subtilis.PloS one · 2017Article
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- Ugp and PitA participate in the selection of PHO-constitutive mutants.Journal of bacteriology · 2015Article
- UvrD helicase: an old dog with a new trick: how one step backward leads to many steps forward.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- Development of a stress-induced mutagenesis module for autonomous adaptive evolution of Escherichia coli to improve its stress tolerance.Biotechnology for biofuels · 2015Article
- Regulation of Transcript Elongation.Annual review of microbiology · 2015Review
- Multiple Pathways of Genome Plasticity Leading to Development of Antibiotic Resistance.Antibiotics (Basel, Switzerland) · 2013Review
- R-loops and nicks initiate DNA breakage and genome instability in non-growing Escherichia coli.Nature communications · 2013Article
- Escherichia coli transcription termination factor NusA: heat-induced oligomerization and chaperone activity.Scientific reports · 2013Article
- Stress-Induced Mutagenesis.EcoSal Plus · 2012Article
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2 authors at 1 institution in 1 country.
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Abstract
Stress-induced mutagenesis describes the accumulation of mutations that occur in nongrowing cells, in contrast to mutagenesis that occurs in actively dividing populations, and has been referred to as stationary-phase or adaptive mutagenesis. The most widely studied system for stress-induced mutagenesis involves monitoring the appearance of Lac(+) revertants of the strain FC40 under starvation conditions in Escherichia coli. The SOS-inducible translesion DNA polymerase DinB plays an important role in this phenomenon. Loss of DinB (DNA pol IV) function results in a severe reduction of Lac(+) revertants. We previously reported that NusA, an essential component of elongating RNA polymerases, interacts with DinB. Here we report our unexpected observation that wild-type NusA function is required for stress-induced mutagenesis. We present evidence that this effect is unlikely to be due to defects in transcription of lac genes but rather is due to an inability to adapt and mutate in response to environmental stress. Furthermore, we extended our analysis to the formation of stress-induced mutants in response to antibiotic treatment, observing the same striking abolition of mutagenesis under entirely different conditions. Our results are the first to implicate NusA as a crucial participant in the phenomenon of stress-induced mutagenesis.
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