ArticleJournal of proteome research2010
Inhibition of protein phosphorylation in MIA pancreatic cancer cells: confluence of metabolic and signaling pathways.
Article in Journal of proteome research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- HMGA1 promotes the progression of esophageal squamous cell carcinoma by elevating TKT-mediated upregulation of pentose phosphate pathway.Cell death & disease · 2024Article
- HCA (2-Hydroxy-Docosahexaenoic Acid) Induces Apoptosis and Endoplasmic Reticulum Stress in Pancreatic Cancer Cells.International journal of molecular sciences · 2022Article
- A dose- and time-dependent effect of oxythiamine on cell growth inhibition in non-small cell lung cancer.Cognitive neurodynamics · 2022Article
- Thiamine and selected thiamine antivitamins - biological activity and methods of synthesis.Bioscience reports · 2018Review
- The Warburg effect: a balance of flux analysis.Metabolomics : Official journal of the Metabolomic Society · 2015Article
- Transketolase is upregulated in metastatic peritoneal implants and promotes ovarian cancer cell proliferation.Clinical & experimental metastasis · 2015Article
- The mechanism of oxythiamine-induced collagen biosynthesis in cultured fibroblasts.Molecular and cellular biochemistry · 2015Article
- Metabolic Consequences of LDHA inhibition by Epigallocatechin Gallate and Oxamate in MIA PaCa-2 Pancreatic Cancer Cells.Metabolomics : Official journal of the Metabolomic Society · 2015Article
- Tissue proteomics in pancreatic cancer study: discovery, emerging technologies, and challenges.Proteomics · 2013Review
- Inhibition of transketolase by oxythiamine altered dynamics of protein signals in pancreatic cancer cells.Experimental hematology & oncology · 2013Article
- Inhibition of glycogen phosphorylation induces changes in cellular proteome and signaling pathways in MIA pancreatic cancer cells.Pancreas · 2012Article
- Concomitant inhibition of HSP90, its mitochondrial localized homologue TRAP1 and HSP27 by green tea in pancreatic cancer HPAF-II cells.Proteomics · 2011Article
- Tracer-based metabolomics: concepts and practices.Clinical biochemistry · 2010Review
Corrections and comments
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Authors and funding
12 authors at 1 institution in 2 countries.
Funding
Abstract
Oxythiamine (OT), a transketolase inhibitor, is known to inhibit pancreatic cancer cell proliferation. In this study, we investigated the effect of inhibition of the transketolase pathway on signaling pathways in MIA PaCa cancer cells using in-house proteomic techniques. We hypothesized that OT alter protein phosphorylation thus affecting cell cycle arrest and cell proliferation. MIA PaCa-2 cells were cultured in media containing an algal (15)N amino acid mixture at 50% enrichment, with and without OT, to determine protein expression and synthesis. Analysis of cell lysates using two-dimensional gel electrophoresis matrix assisted laser desorption and ionization time-of-flight and time-of-flight mass spectrometry (2-DE-MALDI-TOF/TOF MS) identified 12 phosphor proteins that were significantly suppressed by OT treatment. Many of these proteins are involved in regulation of cycle activities and apoptosis. Among the proteins identified, expression of the phosphor heat shock protein 27 (Hsp27) was dramatically inhibited by OT treatment while the level of its total protein remained unchanged. Hsp27 expression and phosphorylation is known to be associated with drug resistance and cancer cell survival. The changes in phosphorylation of key proteins of cancer proliferation and survival suggest that protein phosphorylation is the confluence of the effects of OT on metabolic and signaling pathways.
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