ArticleThe Journal of biological chemistry2010
A comprehensive proteomic analysis of the type III secretome of Citrobacter rodentium.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 74 citations in OpenAlex.
- Substrate secretion by different EHEC secretion systems during their interaction with epithelial cells.Virulence · 2026Article
- The small regulatory RNA DsrA silences the locus of enterocyte effacement of enteropathogenicmicroPublication biology · 2025Article
- Identification of Effectors: Precipitation of Supernatant Material.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- Sialic acid plays a pivotal role in licensingProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Multiparametric Sensing of Outer Membrane Vesicle-Derived Supported Lipid Bilayers Demonstrates the Specificity of Bacteriophage Interactions.ACS biomaterials science & engineering · 2023Article
- Genotoxins: The Mechanistic Links betweenCancers · 2023Review
- Bacterial Genotoxin Accelerates Transient Infection-Driven Murine Colon Tumorigenesis.Cancer discovery · 2022Article
- An acetate-yielding diet imprints an immune and anti-microbial programme against enteric infection.Clinical & translational immunology · 2021Article
- Advances and Challenges in Studying Type III Secretion Effectors of Attaching and Effacing Pathogens.Frontiers in cellular and infection microbiology · 2020Article
- The Prophages ofJournal of bacteriology · 2019Article
- The Evasive Enemy: Insights into the Virulence and Epidemiology of the Emerging Attaching and Effacing PathogenInfection and immunity · 2019Review
- Novel Effector Protein EspY3 of Type III Secretion System from EnterohemorrhagicMicroorganisms · 2018Article
- Host-associated niche metabolism controls enteric infection through fine-tuning the regulation of type 3 secretion.Nature communications · 2018Article
- Systematic Identification of Intracellular-Translocated Candidate Effectors inFrontiers in cellular and infection microbiology · 2018Article
- Identification of a NovelMolecular & cellular proteomics : MCP · 2017Article
- IL-22 Upregulates Epithelial Claudin-2 to Drive Diarrhea and Enteric Pathogen Clearance.Cell host & microbe · 2017Article
- Identification of novel substrates of Shigella T3SA through analysis of its virulence plasmid-encoded secretome.PloS one · 2017Article
- Interference with nuclear factor kappaB signaling pathway by pathogen-encoded proteases: global and selective inhibition.Molecular microbiology · 2016Review
- Comparative Secretome Analysis of Ralstonia solanacearum Type 3 Secretion-Associated Mutants Reveals a Fine Control of Effector Delivery, Essential for Bacterial Pathogenicity.Molecular & cellular proteomics : MCP · 2016Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
Abstract
Enteropathogenic Escherichia coli, enterohemorrhagic E. coli, and Citrobacter rodentium belong to the family of attaching and effacing (A/E) bacterial pathogens. They intimately attach to host intestinal epithelial cells, trigger the effacement of intestinal microvilli, and cause diarrheal disease. Central to their pathogenesis is a type III secretion system (T3SS) encoded by a pathogenicity island called the locus of enterocyte effacement (LEE). The T3SS is used to inject both LEE- and non-LEE-encoded effector proteins into the host cell, where these effectors modulate host signaling pathways and immune responses. Identifying the effectors and elucidating their functions are central to understanding the molecular pathogenesis of these pathogens. Here we analyzed the type III secretome of C. rodentium using the highly sensitive and quantitative SILAC (stable isotope labeling with amino acids in cell culture)-based mass spectrometry. This approach not only confirmed nearly all known secreted proteins and effectors previously identified by conventional biochemical and proteomic techniques, but also identified several new secreted proteins. The T3SS-dependent secretion of these new proteins was validated, and five of them were translocated into cultured cells, representing new or additional effectors. Deletion mutants for genes encoding these effectors were generated in C. rodentium and tested in a murine infection model. This study comprehensively characterizes the type III secretome of C. rodentium, expands the repertoire of type III secreted proteins and effectors for the A/E pathogens, and demonstrates the simplicity and sensitivity of using SILAC-based quantitative proteomics as a tool for identifying substrates for protein secretion systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.