ArticleIn vitro cellular & developmental biology. Animal2010
Newly established cell lines from mouse oral epithelium regenerate teeth when combined with dental mesenchyme.
Article in In vitro cellular & developmental biology. Animal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Retinoic Acid Signalling Regulates Zebrafish Tooth Germ Repair Following Injury.Cell proliferation · 2026Article
- Regenerative teeth induced by in vitro mesenchymal cells in mice via repressing BMP4 and activating retinoic acid/osteopontin.Cell regeneration (London, England) · 2025Article
- Immobilized NRG1 Accelerates Neural Crest like Cell Differentiation Toward Functional Schwann Cells Through Sustained Erk1/2 Activation and YAP/TAZ Nuclear Translocation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- A clonal stem cell line established from a mouse mammary placode with ability to generate functional mammary glands.In vitro cellular & developmental biology. Animal · 2019Article
- Establishment and characterization of immortalized mouse ameloblast-like cell lines.Orthodontics & craniofacial research · 2019Article
- Derivation of neural crest stem cells from human epidermal keratinocytes requires FGF-2, IGF-1, and inhibition of TGF-β1.Bioengineering & translational medicine · 2018Article
- Cell culture models of oral mucosal barriers: A review with a focus on applications, culture conditions and barrier properties.Tissue barriers · 2018Review
- Review
- Test Tube Tooth: The Next Big Thing.Journal of clinical and diagnostic research : JCDR · 2016Review
- Establishment of Hertwig's epithelial root sheath/epithelial rests of Malassez cell line from human periodontium.Molecules and cells · 2014Article
- Article
- Adult human gingival epithelial cells as a source for whole-tooth bioengineering.Journal of dental research · 2013Article
- Characterization of dental epithelial stem cells from the mouse incisor with two-dimensional and three-dimensional platforms.Tissue engineering. Part C, Methods · 2013Article
- Molecular factors resulting in tooth agenesis and contemporary approaches for regeneration: a review.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2012Review
- The benefits and risks of stem cell technology.Oral diseases · 2012Review
- Neural crest stem cells: discovery, properties and potential for therapy.Cell research · 2012Review
- Establishment of clonal cell lines of taste buds from a p53(-/-) mouse tongue.In vitro cellular & developmental biology. Animal · 2011Article
- Disturbed tooth germ development in the absence of MINT in the cultured mouse mandibular explants.Molecular biology reports · 2011Article
- Stem cell-based biological tooth repair and regeneration.Trends in cell biology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study attempted to examine whether clonal cell lines of the oral epithelium can differentiate into ameloblasts and regenerate tooth when combined with dental germ mesenchyme. Clonal cell lines with a distinct morphology were established from the oral epithelium of p53-deficient fetal mice at embryonic day 18 (E18). The strain of mouse is shown to be a useful source for establishing clonal and immortalized cell lines from various tissues and at various stages of development. Tooth morphogenesis is almost completed and the oral epithelium is segregated from the dental epithelium at E18. In RT-PCR analysis of cell lines, mucosal epithelial markers (cytokeratin 14) were detected, but ameloblast markers such as amelogenin and ameloblastin were not detected when cells were cultured on plastic dish. They formed stratified epithelia and expressed a specific differentiation marker (CK13) in the upper layer when cultured on feeder layer or on collagen gel for 1-3 wk, demonstrating that they are of oral mucosa origin. Next, bioengineered tooth germs were prepared with cell lines and fetal molar mesenchymal tissues and implanted under kidney capsule for 2-3 wk. Five among six cell lines regenerated calcified structures as seen in natural tooth. Our results indicate that some oral epithelial cells at E18 possess the capability to differentiate into ameloblasts. Furthermore, cell lines established in the present study are useful models to study processes in tooth organogenesis and tooth regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.