Evidence map›Paper›PMID 20033654›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2010

KIR genotyping by multiplex PCR-SSP.

Smita Kulkarni, Maureen P Martin, Mary Carrington

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 61 citations in OpenAlex.

  1. Trial
  2. Article
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  4. Article
  5. Review
  6. Single Nucleotide Polymorphism inFrontiers in immunology · 2020
    Article
  7. Article
  8. CD8αα homodimers function as a coreceptor for KIR3DL1.Proceedings of the National Academy of Sciences of the United States of America · 2019
    Article
  9. Article
  10. The association ofJournal of Cancer · 2019
    Article
  11. Article
  12. Article
  13. Article
  14. Insights into the Interplay between KIR Gene Frequencies and Chronic HBV Infection in Burkina Faso.Mediterranean journal of hematology and infectious diseases · 2018
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Smita KulkarniCancer and Inflammation Program, Laboratory of Experimental Immunology, SAIC-Frederick Inc., National Cancer Institute-Frederick, Frederick, MD, USA.
Maureen P Martin
Mary Carrington
Science Applications International Corporation (United States) · USNational Cancer Institute · US

Funding

Effects of genetic polymorphism in MHC, KIR, and related loci on human diseaseZIABC010791 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI CARRINGTON, MARY N. · 2009 to 2025
$18.2M
Molecular genetics and population studies of the KIR and HLA gene complexesZIABC010792 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI CARRINGTON, MARY N. · 2009 to 2025
$7.8M
Intramural NIH HHS ZIA BC010791NCI NIH HHS N01 CO012400NCI NIH HHS N01-CO-12400
6 · The paper itself

Abstract

Diversity across KIR haplotypes stems from differences in numbers of inhibitory and activating receptors, as well as allelic polymorphism of individual genes. The KIR locus has undergone large expansions and contractions over time and is believed to be coevolving with genes encoding its HLA class I ligands located within the MHC locus. KIR and HLA compound genotypes have been associated with susceptibility to or protection from infectious, autoimmune, reproductive, and malignant disorders. We describe here a simple and reliable multiplex PCR-SSP (sequence-specific priming) method for relatively rapid and inexpensive genotyping of 15 KIR genes using standard agarose gel electrophoresis.

Indexed as

Base SequenceDNA PrimersElectrophoresis, Agar GelGenotypePolymerase Chain ReactionReceptors, KIRDNA PrimersReceptors, KIR

Identifiers

PMID20033654
PMCPMC3464904
OpenAlexW1519814597

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.