ArticleSexual plant reproduction2009
Mechanism of action of nitrous oxide gas applied as a polyploidizing agent during meiosis in lilies.
Article in Sexual plant reproduction, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Review
- Review
- Effective Induction of Fertile Gametes in Oriental × Trumpet (OT) Lily by High Temperatures.Plants (Basel, Switzerland) · 2023Article
- Haploid induction and its application in maize breeding.Molecular breeding : new strategies in plant improvement · 2021Article
- QTL Mapping and Prediction of Haploid Male Fertility Traits in Maize (Plants (Basel, Switzerland) · 2020Article
- Review
- Doubled haploid technology for line development in maize: technical advances and prospects.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
- Breeding of lilies and tulips-Interspecific hybridization and genetic background.Breeding science · 2018Review
- QTL mapping for haploid male fertility by a segregation distortion method and fine mapping of a key QTL qhmf4 in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- Towards development of new ornamental plants: status and progress in wide hybridization.Planta · 2016Review
- Exploitation of induced 2n-gametes for plant breeding.Plant cell reports · 2014Review
- Organization of actin cytoskeleton during meiosis I in a wheat thermo-sensitive genic male sterile line.Protoplasma · 2013Article
- Multiple mechanisms and challenges for the application of allopolyploidy in plants.International journal of molecular sciences · 2012Review
- N(2)O induces mitotic polyploidization in anther somatic cells and restores fertility in sterile interspecific hybrid lilies.Breeding science · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitrous oxide gas (N(2)O) can be used to produce polyploid plants, but the mechanism of action is unknown. The actin and microtubule cytoskeleton was observed in N(2)O-treated microsporocytes of Lilium spp 'Asiatic hybrid lilies' using fluorescence microscopy after staining with DAPI, FITC-conjugated tubulin antibody, and phalloidin-conjugated Alexa Fluor 546. Additionally, microsporocytes of L. longiflorum were observed with acetocarmine staining following N(2)O treatment. A typical metaphase I microtubule distribution was observed in control microsporocytes. After treatment with N(2)O for 24 h, microtubules were effectively depolymerized; this prevented chromosomes from moving to the poles, resulting in chromosome retention in the center of N(2)O-treated cells. Cell plate formation took place without delay, however, yielding one daughter cell with a diploid genome and another daughter without chromosomes. In addition, N(2)O treatment often induced micronuclei due to aberrant chromosome separation during cytokinesis. Actin filaments in microsporocytes are insensitive to N(2)O. These findings indicate that N(2)O mediates polyploidization by inhibiting microtubule polymerization, but not actin filament formation, during microsporocyte meiosis.
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