ArticleSexual plant reproduction2009
The extracellular lipase EXL4 is required for efficient hydration of Arabidopsis pollen.
Article in Sexual plant reproduction, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
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Who cites it
67 citing papers in PubMed, 127 citations in OpenAlex.
- Assessing the relative contributions of different Arabidopsis thaliana stigma factors to pollen hydration.Plant reproduction · 2026Article
- Knockdown of Arabidopsis RUS4 impacts pollen coat formation by affecting flavonoid and lipid metabolism.Planta · 2026Article
- Molecular understanding of plant pollination, fertilization, seed development and protoplast regeneration.Science China. Life sciences · 2026Review
- ICE1 plays a vital role in the development of pollen grains through regulating tapetum degradation and nutrient mobilization in Arabidopsis thaliana.Plant molecular biology · 2026Article
- ABA-Induced Transcriptomic Dynamics inPlants (Basel, Switzerland) · 2026Article
- Transcriptomic, phenotypic and physiological analyses identify candidate regulators of Dendrobium stigma maturation and pollen-stigma interaction.BMC plant biology · 2025Article
- Insights into the Molecular Basis of Pollen Coat Development and Its Role in Male Sterility.International journal of molecular sciences · 2025Review
- OsPRK1/2/3-mediated reactive oxygen species signaling is required for pollen tube germination in rice.Journal of integrative plant biology · 2025Article
- Genome-wide identification, transcript profiling and functional analyses of PCP gene family in Wucai (Brassica campestris).Scientific reports · 2024Article
- Analysis ofInternational journal of molecular sciences · 2024Article
- Comparative omics-based characterization, phylogeny and melatonin-mediated expression analyses of GDSL genes in pitaya (Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement.Plant reproduction · 2024Review
- Environmental regulation of male fertility is mediated through Arabidopsis transcription factors bHLH89, 91, and 10.Journal of experimental botany · 2024Article
- Genome and GWAS analysis identified genes significantly related to phenotypic state ofHorticulture research · 2024Article
- Identification, evolution, and expression of GDSL-type Esterase/Lipase (GELP) gene family in three cotton species: a bioinformatic analysis.BMC genomics · 2023Article
- Regulatory dynamics of gene expression in the developing male gametophyte of Arabidopsis.Plant reproduction · 2023Article
- Cosuppression of AtGELP22 and AtGELP23, two ubiquitinated target proteins of RING E3 ligase AtAIRP5, increases tolerance to drought stress in Arabidopsis.Plant molecular biology · 2023Article
- Biosynthesis and transport of pollen coat precursors in angiosperms.Nature plants · 2023Review
- Article
- Pollen Coat Proteomes ofBiomolecules · 2023Article
7 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pollination in species with dry stigmas begins with the hydration of desiccated pollen grains on the stigma, a highly regulated process involving the proteins and lipids of the pollen coat and stigma cuticle. Self-incompatible species of the Brassicaceae block pollen hydration, and while the early signaling steps of the self-incompatibility response are well studied, the precise mechanisms controlling pollen hydration are poorly understood. Both lipids and proteins are important for hydration; loss of pollen coat lipids and proteins results in defective or delayed hydration on the stigma surface. Here, we examine the role of the pollen coat protein extracellular lipase 4 (EXL4), in the initial steps of pollination, namely hydration on the stigma. We identify a mutant allele, exl4-1, that shows a reduced rate of pollen hydration. exl4-1 pollen is normal with respect to pollen morphology and the downstream steps in pollination, including pollen tube germination, growth, and fertilization of ovules. However, owing to the delay in hydration, exl4-1 pollen is at a disadvantage when competed with wild-type pollen. EXL4 also functions in combination with GRP17 to promote the initiation of hydration. EXL4 is similar to GDSL lipases, and we show that it functions in hydrolyzing ester bonds. We report a previously unknown function for EXL4, an abundant pollen coat protein, in promoting pollen hydration on the stigma. Our results indicate that changes in lipid composition at the pollen-stigma interface, possibly mediated by EXLs, are required for efficient pollination in species with dry stigmas.
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