ArticleClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology2010
Airway smooth muscle proliferation and survival is not modulated by mast cells.
Article in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Asthmatic Bronchial Matrices Determine the Gene Expression and Behavior of Smooth Muscle Cells in a 3D Culture Model.Frontiers in allergy · 2021Article
- Orosomucoid-like 3 (ORMDL3) upregulates airway smooth muscle proliferation, contraction, and CaThe Journal of allergy and clinical immunology · 2018Article
- Emerging concepts in smooth muscle contributions to airway structure and function: implications for health and disease.American journal of physiology. Lung cellular and molecular physiology · 2016Review
- Mast cells in airway diseases and interstitial lung disease.European journal of pharmacology · 2016Review
- CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- Review
- The role of inflammation resolution speed in airway smooth muscle mass accumulation in asthma: insight from a theoretical model.PloS one · 2014Article
- Airway smooth muscle in airway reactivity and remodeling: what have we learned?American journal of physiology. Lung cellular and molecular physiology · 2013Review
- Laminin drives survival signals to promote a contractile smooth muscle phenotype and airway hyperreactivity.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Origins of increased airway smooth muscle mass in asthma.BMC medicine · 2013Review
- Airway smooth muscle in asthma: just a target for bronchodilation?Clinics in chest medicine · 2012Review
- Abnormal histone methylation is responsible for increased vascular endothelial growth factor 165a secretion from airway smooth muscle cells in asthma.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
- Increased nicotinamide adenine dinucleotide phosphate oxidase 4 expression mediates intrinsic airway smooth muscle hypercontractility in asthma.American journal of respiratory and critical care medicine · 2012Article
- The pivotal role of airway smooth muscle in asthma pathophysiology.Journal of allergy · 2011Article
- Mast cells in allergic asthma and beyond.Yonsei medical journal · 2010Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAirway smooth muscle (ASM) hyperplasia and mast cell localization within the ASM bundle are important features of asthma. The cause of this increased ASM mass is uncertain and whether it is a consequence of ASM-mast cell interactions is unknown.
objectiveWe sought to investigate ASM proliferation and survival in asthma and the effects of co-culture with mast cells.
methodsPrimary ASM cultures were derived from 11 subjects with asthma and 12 non-asthmatic controls. ASM cells were cultured for up to 10 days in the presence or absence of serum either alone or in co-culture with the human mast cell line-1, unstimulated human lung mast cells (HLMC) or IgE/anti-IgE-activated HLMC. Proliferation was assessed by cell counts, CFSE assay and thymidine incorporation. Apoptosis and necrosis were analysed by Annexin V/propidium iodide staining using flow cytometry and by assessment of nuclear morphology using immunofluorescence. Mast cell activation was confirmed by the measurement of histamine release.
resultsUsing a number of techniques, we found that ASM proliferation and survival was not significantly different between cells derived from subjects with or without asthma. Co-culture with mast cells did not affect the rate of proliferation or survival of ASM cells.
conclusionOur findings do not support a role for increased airway smooth proliferation and survival as the major mechanism driving ASM hyperplasia in asthma.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.