ArticleThe Journal of biological chemistry2010
Human negative elongation factor activates transcription and regulates alternative transcription initiation.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- NELFA-mediated pausing restrains YAP transcription and context-dependent outcomes in breast cancer.Frontiers in oncology · 2026Article
- CRISPRi with barcoded expression reporters dissects regulatory networks in human cells.bioRxiv : the preprint server for biology · 2024Article
- The NELF pausing checkpoint mediates the functional divergence of Cdk9.Nature communications · 2023Article
- Dependency of NELF-E-SLUG-KAT2B epigenetic axis in breast cancer carcinogenesis.Nature communications · 2023Article
- Role of NELF-B in supporting epithelial-mesenchymal transition and cell proliferation during hepatocellular carcinoma progression.Oncology letters · 2021Article
- Disorders of sex development in Wolf-Hirschhorn syndrome: a genotype-phenotype correlation and MSX1 as candidate gene.Molecular cytogenetics · 2021Article
- The negative elongation factor NELF promotes induced transcriptional response of Drosophila ecdysone-dependent genes.Scientific reports · 2021Article
- Acute NelfA knockdown restricts compensatory gene expression and precipitates ventricular dysfunction during cardiac hypertrophy.Journal of molecular and cellular cardiology · 2020Article
- NELF Regulates a Promoter-Proximal Step Distinct from RNA Pol II Pause-Release.Molecular cell · 2020Article
- Negative elongation factor is essential for endometrial function.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- The Many Faces ofFrontiers in physiology · 2019Article
- Cytogenomic Integrative Network Analysis of the Critical Region Associated with Wolf-Hirschhorn Syndrome.BioMed research international · 2018Article
- Pause & go: from the discovery of RNA polymerase pausing to its functional implications.Current opinion in cell biology · 2017Review
- Genetic suppression reveals DNA repair-independent antagonism between BRCA1 and COBRA1 in mammary gland development.Nature communications · 2016Article
- RNA polymerase II pausing as a context-dependent reader of the genome.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2016Review
- Histone Deacetylases Positively Regulate Transcription through the Elongation Machinery.Cell reports · 2015Article
- CTCF regulates NELF, DSIF and P-TEFb recruitment during transcription.Transcription · 2015Article
- TRIM28 regulates RNA polymerase II promoter-proximal pausing and pause release.Nature structural & molecular biology · 2014Article
- Negative elongation factor controls energy homeostasis in cardiomyocytes.Cell reports · 2014Article
- Deletions involving genes WHSC1 and LETM1 may be necessary, but are not sufficient to cause Wolf-Hirschhorn Syndrome.European journal of human genetics : EJHG · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The human negative elongation factor (NELF) is a four-subunit protein complex that inhibits the movement of RNA polymerase II (RNAPII) at an early elongation stage in vitro. NELF-mediated stalling of RNAPII also attenuates transcription of a number of inducible genes in human cells. To obtain a genome-wide understanding of human NELF-mediated transcriptional regulation in vivo, we carried out an exon array study in T47D breast cancer cells with transient small interfering RNA knockdown of individual NELF subunits. Upon depletion of NELF-A, -C, or -E, the vast majority of NELF-regulated genes were down-regulated. Many of the down-regulated genes encode proteins that play key roles in cell cycle progression. Consequently, NELF knockdown resulted in significant reduction in DNA synthesis and cell proliferation. Chromatin immunoprecipitation showed that NELF knockdown led to dissociation of RNAPII from the promoter-proximal region of the cell cycle-regulating genes. This was accompanied by decreased histone modifications associated with active transcription initiation (H3K9Ac) and elongation (H3K36Me3), as well as reduced recruitment of the general transcription factor TFIIB and increased overall histone occupancy at a subset of the down-regulated promoters. Lastly, our study indicates that NELF regulates alternative transcription initiation of BSG (Basigin) gene by differentially influencing RNAPII density at the two neighboring exons at the 5' end of the gene. Taken together, our data suggest a diverse transcriptional consequence of NELF-mediated RNAPII pausing in the human genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.