ArticleThe Plant cell2009
Quantitative proteomics of the tonoplast reveals a role for glycolytic enzymes in salt tolerance.
Article in The Plant cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 127 citations in OpenAlex.
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- Identification of Early Salt-Stress-Responsive Proteins in In VitroPlants (Basel, Switzerland) · 2022Article
- Salt stress alters membrane lipid content and lipid biosynthesis pathways in the plasma membrane and tonoplast.Plant physiology · 2022Article
- Reduction of the canonical function of a glycolytic enzyme enolase triggers immune responses that further affect metabolism and growth in Arabidopsis.The Plant cell · 2022Article
- How salt stress-responsive proteins regulate plant adaptation to saline conditions.Plant molecular biology · 2022Review
- Proteomic insight into soybean response to flooding stress reveals changes in energy metabolism and cell wall modifications.PloS one · 2022Article
- Salt stress proteins in plants: An overview.Frontiers in plant science · 2022Review
- Pseudocrossidium replicatum (Taylor) R.H. Zander is a fully desiccation-tolerant moss that expresses an inducible molecular mechanism in response to severe abiotic stress.Plant molecular biology · 2021Article
- Plasma and vacuolar membrane sphingolipidomes: composition and insights on the role of main molecular species.Plant physiology · 2021Article
- Membrane Profiling by Free Flow Electrophoresis and SWATH-MS to Characterize Subcellular Compartment Proteomes inInternational journal of molecular sciences · 2021Article
- Comparative Analysis of Proteins Regulated during Cadmium Sulfide Quantum Dots Response inNanomaterials (Basel, Switzerland) · 2021Article
- Quantitative Proteomics Reveals that GmENO2 Proteins Are Involved in Response to Phosphate Starvation in the Leaves ofInternational journal of molecular sciences · 2021Article
- Plant Proteoforms Under Environmental Stress: Functional Proteins Arising From a Single Gene.Frontiers in plant science · 2021Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To examine the role of the tonoplast in plant salt tolerance and identify proteins involved in the regulation of transporters for vacuolar Na(+) sequestration, we exploited a targeted quantitative proteomics approach. Two-dimensional differential in-gel electrophoresis analysis of free flow zonal electrophoresis separated tonoplast fractions from control, and salt-treated Mesembryanthemum crystallinum plants revealed the membrane association of glycolytic enzymes aldolase and enolase, along with subunits of the vacuolar H(+)-ATPase V-ATPase. Protein blot analysis confirmed coordinated salt regulation of these proteins, and chaotrope treatment indicated a strong tonoplast association. Reciprocal coimmunoprecipitation studies revealed that the glycolytic enzymes interacted with the V-ATPase subunit B VHA-B, and aldolase was shown to stimulate V-ATPase activity in vitro by increasing the affinity for ATP. To investigate a physiological role for this association, the Arabidopsis thaliana cytoplasmic enolase mutant, los2, was characterized. These plants were salt sensitive, and there was a specific reduction in enolase abundance in the tonoplast from salt-treated plants. Moreover, tonoplast isolated from mutant plants showed an impaired ability for aldolase stimulation of V-ATPase hydrolytic activity. The association of glycolytic proteins with the tonoplast may not only channel ATP to the V-ATPase, but also directly upregulate H(+)-pump activity.
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