ArticleThe Plant cell2009
Heat shock protein cognate 70-4 and an E3 ubiquitin ligase, CHIP, mediate plastid-destined precursor degradation through the ubiquitin-26S proteasome system in Arabidopsis.
Article in The Plant cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed, 196 citations in OpenAlex.
- Chloroplast Protein Degradation Pathways During Leaf Senescence: Coordination and Roles in Nitrogen Remobilization.Plants (Basel, Switzerland) · 2026Review
- Dual Cytoplasmic and Chloroplastic Mechanisms Fine-Tune Chloroplast Division through ARC3 Protein Stability.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The transcription factor ANAC017 links mitochondrial retrograde signaling with the ubiquitin-proteasome system to control mitochondrial function in Arabidopsis.Plant communications · 2026Article
- Proteostasis and protein quality control in chloroplasts: mechanisms and novel insights related to protein mislocalization.Journal of experimental botany · 2025Review
- A tubby-like protein, MdTLP7 enhances drought and salt stresses tolerance of Malus domestica.BMC plant biology · 2025Article
- Chloroplast precursor protein preClpD overaccumulation triggers multilevel reprogramming of gene expression and a heat shock-like response.Nature communications · 2025Article
- Transcriptomic and proteomic-based analysis of the mechanisms by which drought and salt stresses affect the quality of Isatidis Folium.BMC plant biology · 2025Article
- Genome-Wide Identification of theInternational journal of molecular sciences · 2024Article
- Protein degrons and degradation: Exploring substrate recognition and pathway selection in plants.The Plant cell · 2024Review
- The lowdown on breakdown: Open questions in plant proteolysis.The Plant cell · 2024Review
- Chloroplast protein StFC-II was manipulated by aHorticulture research · 2024Article
- Chloroplast protein translocation pathways and ubiquitin-dependent regulation at a glance.Journal of cell science · 2023Article
- Protein Profiling ofInternational journal of molecular sciences · 2023Article
- Selective autophagy regulates chloroplast protein import and promotes plant stress tolerance.The EMBO journal · 2023Article
- UPL5 modulates WHY2 protein distribution in a Kub-site dependent ubiquitination in response to [CaiScience · 2023Article
- Research progress on maintaining chloroplast homeostasis under stress conditions: a review.Acta biochimica et biophysica Sinica · 2023Review
- Chloroplast proteostasis: A story of birth, life, and death.Plant communications · 2023Review
- Ubiquitin-based pathway acts inside chloroplasts to regulate photosynthesis.Science advances · 2022Article
- Chaperone-assisted E3 ligase CHIP: A double agent in cancer.Genes & diseases · 2022Review
- Chloroplast protein import machinery and quality control.The FEBS journal · 2022Review
36 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plastid-targeted proteins pass through the cytosol as unfolded precursors. If proteins accumulate in the cytosol, they can form nonspecific aggregates that cause severe cellular damage. Here, we demonstrate that high levels of plastid precursors are degraded through the ubiquitin-proteasome system (UPS) in Arabidopsis thaliana cells. The cytosolic heat shock protein cognate 70-4 (Hsc70-4) and E3 ligase carboxy terminus of Hsc70-interacting protein (CHIP) were highly induced in plastid protein import2 plants, which had a T-DNA insertion at Toc159 and showed an albino phenotype and a severe defect in protein import into chloroplasts. Hsc70-4 and CHIP together mediated plastid precursor degradation when import-defective chloroplast-targeted reporter proteins were transiently expressed in protoplasts. Hsc70-4 recognized specific sequence motifs in transit peptides and thereby led to precursor degradation through the UPS. CHIP, which interacted with Hsc70-4, functioned as an E3 ligase in the Hsc70-4-mediated protein degradation. The physiological role of Hsc70-4 was confirmed by analyzing Hsc70-4 RNA interference plants in an hsc70-1 mutant background. Plants with lower Hsc70 levels exhibited abnormal embryogenesis, resulting in defective seedlings that displayed high levels of reactive oxygen species and monoubiquitinated Lhcb4 precursors. We propose that Hsc70-4 and CHIP mediate plastid-destined precursor degradation to prevent cytosolic precursor accumulation and thereby play a critical role in embryogenesis.
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