ArticleInfection and immunity2010
The Pseudomonas aeruginosa magnesium transporter MgtE inhibits transcription of the type III secretion system.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 45 citations in OpenAlex.
- Characterization of hypothetical proteins reveals function modules and virulence factors in Pseudomonas aeruginosa.Nature communications · 2026Article
- TypA Interacts with ExsA to Suppress Type III Secretion System inMicroorganisms · 2026Article
- Genomic and phenotypic diversification ofMicrobiology spectrum · 2026Article
- Magnesium Transporters as Crucial Regulators of Bacterial Survival and Pathogenicity.Microorganisms · 2026Review
- Subgingival microbiome in periodontitis and type 2 diabetes mellitus: an exploratory study using metagenomic sequencing.Journal of periodontal & implant science · 2022Article
- Sub-Inhibitory Antibiotic Exposure and Virulence inAntibiotics (Basel, Switzerland) · 2021Review
- cAMP Receptor Protein Positively Regulates the Expression of Genes Involved in the Biosynthesis ofFrontiers in microbiology · 2021Article
- NrtR Regulates the Type III Secretion System Through cAMP/Vfr Pathway inFrontiers in microbiology · 2019Article
- Label-Free Proteomics of a Defined, Binary Co-culture Reveals Diversity of Competitive Responses Between Members of a Model Soil Microbial System.Microbial ecology · 2018Article
- Pseudomonas aeruginosa Magnesium Transporter MgtE Inhibits Type III Secretion System Gene Expression by StimulatingJournal of bacteriology · 2017Article
- Fis Regulates Type III Secretion System by Influencing the Transcription ofFrontiers in microbiology · 2017Article
- Mycoplasma non-coding RNA: identification of small RNAs and targets.BMC genomics · 2016Article
- Pan-genome dynamics of Pseudomonas gene complements enriched across hexachlorocyclohexane dumpsite.BMC genomics · 2015Article
- Impact of alginate-producing Pseudomonas aeruginosa on alveolar macrophage apoptotic cell clearance.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2015Article
- Acidosis potentiates the host proinflammatory interleukin-1β response to Pseudomonas aeruginosa infection.Infection and immunity · 2014Article
- The global regulator Crc plays a multifaceted role in modulation of type III secretion system in Pseudomonas aeruginosa.MicrobiologyOpen · 2013Article
- Eradication of Pseudomonas aeruginosa biofilms on cultured airway cells by a fosfomycin/tobramycin antibiotic combination.Pathogens and disease · 2013Article
- Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2013Article
- Bacterial Mg2+ homeostasis, transport, and virulence.Annual review of genetics · 2013Review
- Response of P. aeruginosa E(1) gene expression to cadmium stress.Current microbiology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Pseudomonas aeruginosa is an opportunistic pathogen that causes life-long pneumonia in individuals with cystic fibrosis (CF). These long-term infections are maintained by bacterial biofilm formation in the CF lung. We have recently developed a model of P. aeruginosa biofilm formation on cultured CF airway epithelial cells. Using this model, we discovered that mutation of a putative magnesium transporter gene, called mgtE, led to increased cytotoxicity of P. aeruginosa toward epithelial cells. This altered toxicity appeared to be dependent upon expression of the type III secretion system (T3SS). In this study, we found that mutation of mgtE results in increased T3SS gene transcription. Through epistasis analyses, we discovered that MgtE influences the ExsE-ExsC-ExsD-ExsA gene regulatory system of T3SS by either directly or indirectly inhibiting ExsA activity. While variations in calcium levels modulate T3SS gene expression in P. aeruginosa, we found that addition of exogenous magnesium did not inhibit T3SS activity. Furthermore, mgtE variants that were defective for magnesium transport could still complement the cytotoxicity effect. Thus, the magnesium transport function of MgtE does not fully explain the regulatory effects of MgtE on cytotoxicity. Overall, our results indicate that MgtE modulates expression of T3SS genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.