ArticleMolecular and cellular biology2010
Splicing factor Spf30 assists exosome-mediated gene silencing in fission yeast.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 24 citations in OpenAlex.
- Fungi as models of centromere innovation: from DNA sequence to 3-dimensional arrangement.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- The role of Arabidopsis Splicing Factor 30 in floral transition and the implications of its alternative splicing.Plant physiology · 2025Article
- Splicing of branchpoint-distant exons is promoted by Cactin, Tls1 and the ubiquitin-fold-activated Sde2.Nucleic acids research · 2022Article
- The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin.PLoS genetics · 2017Article
- Collaborative Control of Cell Cycle Progression by the RNA Exonuclease Dis3 and Ras Is Conserved Across Species.Genetics · 2016Article
- The expanding world of small RNAs in plants.Nature reviews. Molecular cell biology · 2015Review
- Epigenetic Regulation of Chromatin States in Schizosaccharomyces pombe.Cold Spring Harbor perspectives in biology · 2015Review
- Tls1 regulates splicing of shelterin components to control telomeric heterochromatin assembly and telomere length.Nucleic acids research · 2014Article
- Arabidopsis RRP6L1 and RRP6L2 function in FLOWERING LOCUS C silencing via regulation of antisense RNA synthesis.PLoS genetics · 2014Article
- CPF-associated phosphatase activity opposes condensin-mediated chromosome condensation.PLoS genetics · 2014Article
- A genetic screen for functional partners of condensin in fission yeast.G3 (Bethesda, Md.) · 2014Article
- The proper splicing of RNAi factors is critical for pericentric heterochromatin assembly in fission yeast.PLoS genetics · 2014Article
- The spliceosome as a transposon sensor.RNA biology · 2013Review
- The splicing machinery promotes RNA-directed DNA methylation and transcriptional silencing in Arabidopsis.The EMBO journal · 2013Article
- The role of the Arabidopsis Exosome in siRNA-independent silencing of heterochromatic loci.PLoS genetics · 2013Article
- Article
- Different means, same end-heterochromatin formation by RNAi and RNAi-independent RNA processing factors in fission yeast.Current opinion in genetics & development · 2012Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterochromatin assembly in fission yeast relies on the processing of cognate noncoding RNAs by both the RNA interference and the exosome degradation pathways. Recent evidence indicates that splicing factors facilitate the cotranscriptional processing of centromeric transcripts into small interfering RNAs (siRNAs). In contrast, how the exosome contributes to heterochromatin assembly and whether it also relies upon splicing factors were unknown. We provide here evidence that fission yeast Spf30 is a splicing factor involved in the exosome pathway of heterochromatin silencing. Spf30 and Dis3, the main exosome RNase, colocalize at centromeric heterochromatin and euchromatic genes. At the centromeres, Dis3 helps recruiting Spf30, whose deficiency phenocopies the dis3-54 mutant: heterochromatin is impaired, as evidenced by reduced silencing and the accumulation of polyadenylated centromeric transcripts, but the production of siRNAs appears to be unaffected. Consistent with a direct role, Spf30 binds centromeric transcripts and locates at the centromeres in an RNA-dependent manner. We propose that Spf30, bound to nascent centromeric transcripts, perhaps with other splicing factors, assists their processing by the exosome. Splicing factor intercession may thus be a common feature of gene silencing pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.