ArticlePLoS biology2009
Transglutaminase-mediated semen coagulation controls sperm storage in the malaria mosquito.
Article in PLoS biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed, 130 citations in OpenAlex.
- Article
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- A knockdown in the expression of a male-specific aminopeptidase impedes the male reproductive success of the mosquitoPhysiological entomology · 2025Article
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- Maternal lipid mobilization is essential for embryonic development in the malaria vector Anopheles gambiae.PLoS biology · 2024Article
- Gone but not forgotten: dynamics of sperm storage and potential ejaculate digestion in the black soldier flyRoyal Society open science · 2024Article
- Eliminating malaria vectors with precision-guided sterile males.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Proteomic diversification of spermatostyles among six species of whirligig beetles.Molecular reproduction and development · 2024Article
- Eliminating Malaria Vectors with Precision Guided Sterile Males.bioRxiv : the preprint server for biology · 2023Article
- Selection for insecticide resistance can promote Plasmodium falciparum infection in Anopheles.PLoS pathogens · 2023Article
- Silencing Transglutaminase GenesInsects · 2022Article
- Sexual transmission of Anopheles gambiae densovirus (AgDNV) leads to disseminated infection in mated females.Parasites & vectors · 2022Article
- Transglutaminase 3 negatively regulates immune responses on the heart of the mosquito, Anopheles gambiae.Scientific reports · 2022Article
- Swarming Behavior in Anopheles gambiae (sensu lato): Current Knowledge and Future Outlook.Journal of medical entomology · 2022Review
- Review
- Intraspecific Transcriptome Variation and Sex-Biased Expression in Anopheles arabiensis.Genome biology and evolution · 2021Article
- Factors Influencing the Reproductive Ability of Male Bees: Current Knowledge and Further Directions.Insects · 2021Review
- Mating-regulated atrial proteases control reinsemination rates in Anopheles gambiae females.Scientific reports · 2020Article
- JNK signaling regulates oviposition in the malaria vector Anopheles gambiae.Scientific reports · 2020Article
- Injection of seminal fluid into the hemocoel of honey bee queens (Apis mellifera) can stimulate post-mating changes.Scientific reports · 2020Article
23 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Insect seminal fluid proteins are powerful modulators of many aspects of female physiology and behaviour including longevity, egg production, sperm storage, and remating. The crucial role of these proteins in reproduction makes them promising targets for developing tools aimed at reducing the population sizes of vectors of disease. In the malaria mosquito Anopheles gambiae, seminal secretions produced by the male accessory glands (MAGs) are transferred to females in the form of a coagulated mass called the mating plug. The potential of seminal fluid proteins as tools for mosquito control demands that we improve our limited understanding of the composition and function of the plug. Here, we show that the plug is a key determinant of An. gambiae reproductive success. We uncover the composition of the plug and demonstrate it is formed through the cross-linking of seminal proteins mediated by a MAG-specific transglutaminase (TGase), a mechanism remarkably similar to mammalian semen coagulation. Interfering with TGase expression in males inhibits plug formation and transfer, and prevents females from storing sperm with obvious consequences for fertility. Moreover, we show that the MAG-specific TGase is restricted to the anopheline lineage, where it functions to promote sperm storage rather than as a mechanical barrier to re-insemination. Taken together, these data represent a major advance in our understanding of the factors shaping Anopheles reproductive biology.
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Registered trials
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