ArticleGenetics2010
Mutational analysis of Candida albicans SNF7 reveals genetically separable Rim101 and ESCRT functions and demonstrates divergence in bro1-domain protein interactions.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Morphology Changes in Human Fungal Pathogens upon Interaction with the Host.Journal of fungi (Basel, Switzerland) · 2017Article
- Extracellular vesicles and infectious diseases: new complexity to an old story.The Journal of clinical investigation · 2016Review
- The endosomal sorting complex required for transport machinery influences haem uptake and capsule elaboration in Cryptococcus neoformans.Molecular microbiology · 2015Article
- The vacuolar-sorting protein Snf7 is required for export of virulence determinants in members of the Cryptococcus neoformans complex.Scientific reports · 2014Article
- pH signaling in human fungal pathogens: a new target for antifungal strategies.Eukaryotic cell · 2014Review
- Interaction maps of the Saccharomyces cerevisiae ESCRT-III protein Snf7.Eukaryotic cell · 2013Article
- Shuttle vectors for facile gap repair cloning and integration into a neutral locus in Candida albicans.Microbiology (Reading, England) · 2013Article
- The β-arrestin-like protein Rim8 is hyperphosphorylated and complexes with Rim21 and Rim101 to promote adaptation to neutral-alkaline pH.Eukaryotic cell · 2012Article
- Rescue of Aspergillus nidulans severely debilitating null mutations in ESCRT-0, I, II and III genes by inactivation of a salt-tolerance pathway allows examination of ESCRT gene roles in pH signalling.Journal of cell science · 2011Article
- Cryptococcal titan cell formation is regulated by G-protein signaling in response to multiple stimuli.Eukaryotic cell · 2011Article
- The Candida albicans ESCRT pathway makes Rim101-dependent and -independent contributions to pathogenesis.Eukaryotic cell · 2010Article
- Intervention of Bro1 in pH-responsive Rim20 localization in Saccharomyces cerevisiae.Eukaryotic cell · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The opportunistic pathogen Candida albicans can grow over a wide pH range, which is associated with its ability to colonize and infect distinct host niches. C. albicans growth in neutral-alkaline environments requires proteolytic activation of the transcription factor Rim101. Rim101 activation requires Snf7, a member of the endosomal sorting complex required for transport (ESCRT) pathway. We hypothesized that Snf7 has distinct functions in the Rim101 and ESCRT pathways, which we tested by alanine-scanning mutagenesis. While some snf7 alleles conferred no defects, we identified alleles with solely ESCRT-dependent, solely Rim101-dependent, or both Rim101- and ESCRT-dependent defects. Thus, Snf7 function in these two pathways is at least partially separable. Both Rim101- and ESCRT-dependent functions require Snf7 recruitment to the endosomal membrane and alleles that disrupted both pathways were found to localize normally, suggesting a downstream defect. Most alleles that conferred solely Rim101-dependent defects were still able to process Rim101 normally under steady-state conditions. However, these same strains did display a kinetic defect in Rim101 processing. Several alleles with solely Rim101-dependent defects mapped to the C-terminal end of Snf7. Further analyses suggested that these mutations disrupted interactions with bro-domain proteins, Rim20 and Bro1, in overlapping but slightly divergent Snf7 domains.
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