ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2010
Synthesis and organization of hyaluronan and versican by embryonic stem cells undergoing embryoid body differentiation.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 54 citations in OpenAlex.
- V3: an enigmatic isoform of the proteoglycan versican.American journal of physiology. Cell physiology · 2023Review
- In Vitro 3D Modeling of Neurodegenerative Diseases.Bioengineering (Basel, Switzerland) · 2023Review
- Activin A promotes hyaluronan production and upregulates versican expression in human granulosa cells†.Biology of reproduction · 2022Article
- Rg3 and Rh2 ginsenosides suppress embryoid body formation by inhibiting the epithelial-mesenchymal transition.Archives of pharmacal research · 2022Article
- The versican-hyaluronan complex provides an essential extracellular matrix niche for Flk1Matrix biology : journal of the International Society for Matrix Biology · 2021Article
- Comparison of the formation, adipogenic maturation, and retention of human adipose-derived stem cell spheroids in scaffold-free culture techniques.Journal of biomedical materials research. Part B, Applied biomaterials · 2020Article
- Up-regulation of EMT-related gene VCAN by NPM1 mutant-driven TGF-β/cPML signalling promotes leukemia cell invasion.Journal of Cancer · 2019Article
- Effect of cellular mass on chondrogenic differentiation during embryoid body formation.Molecular medicine reports · 2018Article
- Provisional matrix: A role for versican and hyaluronan.Matrix biology : journal of the International Society for Matrix Biology · 2017Review
- A Trickster in Disguise: Hyaluronan's Ambivalent Roles in the Matrix.Frontiers in oncology · 2017Review
- Expression of a hyaluronic acid-binding proteoglycan (versican) in the cynomolgus monkey eye.International ophthalmology · 2016Article
- Versican accumulates in vascular lesions in pulmonary arterial hypertension.Pulmonary circulation · 2016Article
- Hyaluronan Is Crucial for Stem Cell Differentiation into Smooth Muscle Lineage.Stem cells (Dayton, Ohio) · 2016Article
- Differential Proteomic Analysis of Human Placenta-Derived Mesenchymal Stem Cells Cultured on Normal Tissue Culture Surface and Hyaluronan-Coated Surface.Stem cells international · 2016Article
- Correlation of Versican Expression, Accumulation, and Degradation during Embryonic Development by Quantitative Immunohistochemistry.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2015Article
- Cell-derived matrices for tissue engineering and regenerative medicine applications.Biomaterials science · 2015Article
- Intrathecal delivery of mesenchymal stromal cells protects the structure of altered perineuronal nets in SOD1 rats and amends the course of ALS.Stem cells (Dayton, Ohio) · 2014Article
- Tissue engineering and regenerative repair in wound healing.Annals of biomedical engineering · 2014Review
- Mesenchymal morphogenesis of embryonic stem cells dynamically modulates the biophysical microtissue niche.Scientific reports · 2014Article
- Soluble factors secreted by differentiating embryonic stem cells stimulate exogenous cell proliferation and migration.Stem cell research & therapy · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
Abstract
Embryonic stem cells (ESCs) provide a convenient model to probe the molecular and cellular dynamics of developmental cell morphogenesis. ESC differentiation in vitro via embryoid bodies (EBs) recapitulates many aspects of early stages of development, including the epithelial-mesenchymal transition (EMT) of pluripotent cells into more differentiated progeny. Hyaluronan and versican are important extracellular mediators of EMT processes, yet the temporal expression and spatial distribution of these extracellular matrix (ECM) molecules during EB differentiation remains undefined. Thus, the objective of this study was to evaluate the synthesis and organization of hyaluronan and versican by using murine ESCs during EB differentiation. Hyaluronan and versican (V0 and V1 isoforms), visualized by immunohistochemistry and evaluated biochemically, accumulated within EBs during the course of differentiation. Interestingly, increasing amounts of a 70-kDa proteolytic fragment of versican were also detected over time, along with ADAMTS-1 and -5 protein expression. ESCs expressed each of the hyaluronan synthases (HAS) -1, -2, and -3 and versican splice variants (V0, V1, V2, and V3) throughout EB differentiation, but HAS-2, V0, and V1 were expressed at significantly increased levels at each time point examined. Hyaluronan and versican exhibited overlapping expression patterns within EBs in regions of low cell density, and versican expression was excluded from clusters of epithelial (cytokeratin-positive) cells but was enriched within the vicinity of mesenchymal (N-cadherin-positive) cells. These results indicate that hyaluronan and versican synthesized by ESCs within EB microenvironments are associated with EMT processes and furthermore suggest that endogenously produced ECM molecules play a role in ESC differentiation. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.