ArticleThe Journal of biological chemistry2010
A calcium/calmodulin-regulated member of the receptor-like kinase family confers cold tolerance in plants.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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93 citing papers in PubMed, 240 citations in OpenAlex.
- Temperature regulation in plants: From molecular mechanisms to climate-resilient crop improvement.Journal of integrative plant biology · 2026Review
- The receptor-like pseudokinase LENG stimulates chilling tolerance in rice by inhibiting the activity of the OsCRPK1-OsGF14d module.Journal of integrative plant biology · 2026Article
- Plant responses to cold stress: molecular and physiological adaptive mechanisms with breeding advances.Frontiers in plant science · 2026Review
- Memory in the wall: expanding our understanding of the roles of plant cell walls.The New phytologist · 2026Review
- Unconventional binding of calmodulin to CHK2 kinase inhibits catalytic activity.The Biochemical journal · 2025Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- Lonicera caerulea genome reveals molecular mechanisms of freezing tolerance and anthocyanin biosynthesis.Journal of advanced research · 2025Article
- Molecular Networks Governing Plant Responses to Heat and Cold Stress.Plants (Basel, Switzerland) · 2025Review
- A receptor-kinase cascade confers cold-induced root growth inhibition in Arabidopsis.Nature plants · 2025Article
- Genome-Wide Identification and Functional Prediction of LRR-RLK Family Genes in Foxtail Millet (International journal of molecular sciences · 2025Article
- Identification of the Plant Defensin (MsPDF) Gene Family inPlants (Basel, Switzerland) · 2025Article
- Integration of GWAS and transcriptome analysis to identify temperature-dependent genes involved in germination of rapeseed (Frontiers in plant science · 2025Article
- Candidate genes associated with low temperature tolerance in cucumber adult plants identified by combining GWAS & QTL mapping.Stress biology · 2024Article
- A Genome-Wide Characterization of Receptor-like Cytoplasmic Kinase IV Subfamily Members inPlants (Basel, Switzerland) · 2024Article
- Differential phosphorylation of Ca2+-permeable channel CYCLIC NUCLEOTIDE-GATED CHANNEL20 modulates calcium-mediated freezing tolerance in Arabidopsis.The Plant cell · 2024Article
- Molecular and biochemical components associated with chilling tolerance in tomato: comparison of different developmental stages.Molecular horticulture · 2024Article
- Insights into Plant Sensory Mechanisms under Abiotic Stresses.Plants (Basel, Switzerland) · 2024Review
- A clade of receptor-like cytoplasmic kinases and 14-3-3 proteins coordinate inositol hexaphosphate accumulation.Nature communications · 2024Article
- Regulatory networks in plant responses to drought and cold stress.Plant physiology · 2024Review
- Discovery of gene regulation mechanisms associated with uniconazole-induced cold tolerance in banana using integrated transcriptome and metabolome analysis.BMC plant biology · 2024Article
33 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cold is a limiting environmental factor that adversely affects plant growth and productivity. Calcium/calmodulin-mediated signaling is believed to play a pivotal role in plant response to cold stress, but its exact role is not clearly understood. Here, we report that CRLK1, a novel calcium/calmodulin-regulated receptor-like kinase, is crucial for cold tolerance in plants. CRLK1 has two calmodulin-binding sites with different affinities as follows: one located at residues 369-390 with a K(d) of 25 nm, and the other located at residues 28-112 with a K(d) of 160 nm. Calcium/calmodulin stimulated the kinase activity, but the addition of chlorpromazine, a calmodulin antagonist, blocked its stimulation. CRLK1 is mainly localized in the plasma membrane, and its expression is stimulated by cold and hydrogen peroxide treatments. Under normal growth conditions, there is no noticeable phenotypic difference between wild-type and crlk1 knock-out mutant plants. However, as compared with wild-type plants, the crlk1 knock-out mutants exhibited an increased sensitivity to chilling and freezing temperatures. Northern analysis showed that the induction of cold-responsive genes, including CBF1, RD29A, COR15a, and KIN1 in crlk1 mutants, is delayed as compared with wild-type plants. These results indicate that CRLK1 is a positive regulator of cold tolerance in plants. Furthermore, our results suggest that CRLK1 plays a role in bridging calcium/calmodulin signaling and cold signaling.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.