ArticleThe Journal of biological chemistry2010
Vesicle-associated membrane protein (VAMP) cleavage by a new metalloprotease from the Brazilian scorpion Tityus serrulatus.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 83 citations in OpenAlex.
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- An overview of some enzymes from buthid scorpion venoms from Colombia:The journal of venomous animals and toxins including tropical diseases · 2024Article
- Understanding the complexity ofThe journal of venomous animals and toxins including tropical diseases · 2024Review
- Scorpion Venom as a Source of Antimicrobial Peptides: Overview of Biomolecule Separation, Analysis and Characterization Methods.Antibiotics (Basel, Switzerland) · 2023Review
- The Enzymatic Core of Scorpion Venoms.Toxins · 2022Article
- Comparison of the Scorpionism Caused byToxins · 2021Article
- Comparison of biological activities ofThe journal of venomous animals and toxins including tropical diseases · 2021Article
- Genetic and toxinological divergence among populations of Tityus trivittatus Kraepelin, 1898 (Scorpiones: Buthidae) inhabiting Paraguay and Argentina.PLoS neglected tropical diseases · 2020Article
- Scorpion Venom: Detriments and Benefits.Biomedicines · 2020Review
- Dissecting Toxicity: The Venom Gland Transcriptome and the Venom Proteome of the Highly Venomous ScorpionToxins · 2019Article
- Purification and Biochemical Characterization of TsMS 3 and TsMS 4: Neuropeptide-Degrading Metallopeptidases in theToxins · 2019Article
- Proteome of fraction fromThe journal of venomous animals and toxins including tropical diseases · 2019Article
- Proteomic endorsed transcriptomic profiles of venom glands from Tityus obscurus and T. serrulatus scorpions.PloS one · 2018Article
- Sequestration of synaptic proteins by alpha-synuclein aggregates leading to neurotoxicity is inhibited by small peptide.PloS one · 2018Article
- A Deeper Examination of Thorellius atrox Scorpion Venom Components with Omic Techonologies.Toxins · 2017Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
We present evidence that venom from the Brazilian scorpion Tityus serrulatus and a purified fraction selectively cleave essential SNARE proteins within exocrine pancreatic tissue. Western blotting for vesicle-associated membrane protein type v-SNARE proteins (or synaptobrevins) reveals characteristic alterations to venom-treated excised pancreatic lobules in vitro. Immunocytochemistry by electron microscopy confirms both the SNARE identity as VAMP2 and the proteolysis of VAMP2 as a marked decrease in secondary antibody-conjugated colloidal gold particles that are predominantly associated with mature zymogen granules. Studies with recombinant SNARE proteins were used to determine the specific cleavage site in VAMP2 and the susceptibility of VAMP8 (endobrevin). The VAMP2 cleavage site is between the transmembrane anchor and the SNARE motif that assembles into the ternary SNARE complex. Inclusion of divalent chelating agents (EDTA) with fraction nu, an otherwise active purified component from venom, eliminates SNARE proteolysis, suggesting the active protein is a metalloprotease. The unique cleavages of VAMP2 and VAMP8 may be linked to pancreatitis that develops following scorpion envenomation as both of these v-SNARE proteins are associated with zymogen granule membranes in pancreatic acinar cells. We have isolated antarease, a metalloprotease from fraction nu that cleaves VAMP2, and report its amino acid sequence.
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