ArticleNucleic acids research2010
Generation of redesigned homing endonucleases comprising DNA-binding domains derived from two different scaffolds.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed.
- AAV-mediated gene therapies by miniature gene editing tools.Science China. Life sciences · 2024Review
- Recent Advances in Gene Therapy for Familial Hypercholesterolemia: An Update Review.Journal of clinical medicine · 2022Review
- Gene Editing for Inherited Red Blood Cell Diseases.Frontiers in physiology · 2022Review
- Genome-editing approaches and applications: a brief review on CRISPR technology and its role in cancer.3 Biotech · 2021Review
- Meganuclease targeting of PCSK9 in macaque liver leads to stable reduction in serum cholesterol.Nature biotechnology · 2018Article
- Programmable Genome Editing Tools and their Regulation for Efficient Genome Engineering.Computational and structural biotechnology journal · 2017Review
- CRISPR/Cas9 in Genome Editing and Beyond.Annual review of biochemistry · 2016Review
- Recent advances in developing molecular tools for targeted genome engineering of mammalian cells.BMB reports · 2015Review
- Efficient design of meganucleases using a machine learning approach.BMC bioinformatics · 2014Article
- DNA cleavage enzymes for treatment of persistent viral infections: recent advances and the pathway forward.Virology · 2014Review
- Homing endonucleases: from genetic anomalies to programmable genomic clippers.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Crystallization and preliminary crystallographic analysis of an Escherichia coli-selected mutant of the nuclease domain of the metallonuclease colicin E7.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- Targeted DNA excision in Arabidopsis by a re-engineered homing endonuclease.BMC biotechnology · 2012Article
- Engineering domain fusion chimeras from I-OnuI family LAGLIDADG homing endonucleases.Nucleic acids research · 2012Article
- LAHEDES: the LAGLIDADG homing endonuclease database and engineering server.Nucleic acids research · 2012Article
- Expanding LAGLIDADG endonuclease scaffold diversity by rapidly surveying evolutionary sequence space.Nucleic acids research · 2012Article
- Development of safer gene delivery systems to minimize the risk of insertional mutagenesis-related malignancies: a critical issue for the field of gene therapy.ISRN oncology · 2012Article
- Manipulating piggyBac transposon chromosomal integration site selection in human cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Tapping natural reservoirs of homing endonucleases for targeted gene modification.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Context dependence between subdomains in the DNA binding interface of the I-CreI homing endonuclease.Nucleic acids research · 2011Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homing endonucleases have become valuable tools for genome engineering. Their sequence recognition repertoires can be expanded by modifying their specificities or by creating chimeric proteins through domain swapping between two subdomains of different homing endonucleases. Here, we show that these two approaches can be combined to create engineered meganucleases with new specificities. We demonstrate the modularity of the chimeric DmoCre meganuclease previously described, by successfully assembling mutants with locally altered specificities affecting both I-DmoI and I-CreI subdomains in order to create active meganucleases with altered specificities. Moreover these new engineered DmoCre variants appear highly specific and present a low toxicity level, similar to I-SceI, and can induce efficient homologous recombination events in mammalian cells. The DmoCre based meganucleases can therefore offer new possibilities for various genome engineering applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.