ArticleToxicology and applied pharmacology2010
Use of human bronchial epithelial cells (BEAS-2B) to study immunological markers resulting from exposure to PM(2.5) organic extract from Puerto Rico.
Article in Toxicology and applied pharmacology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 84 citations in OpenAlex.
- Anti-Inflammatory Properties of Agarwood (Aquilaria Spp.): An Integrated Review of Phytochemistry, Molecular Mechanisms, and Therapeutic Applications.Drug design, development and therapy · 2026Review
- The regulatory role of ACP5 in the diesel exhaust particle-induced AHR inflammatory signaling pathway in a human bronchial epithelial cell line.Scientific reports · 2025Article
- Wuhan strain of SARS-CoV-2 triggers activation of immune evasion machinery similar to the one operated by cancer cells.Frontiers in immunology · 2025Article
- The chemical composition of secondary organic aerosols regulates transcriptomic and metabolomic signaling in an epithelial-endothelial in vitro coculture.Particle and fibre toxicology · 2024Article
- PMToxics · 2024Article
- The biological interplay between air pollutants and miRNAs regulation in cancer.Frontiers in cell and developmental biology · 2024Review
- Review
- Isosinensetin alleviates the injury of human bronchial epithelial cells induced by PMExperimental and therapeutic medicine · 2021Article
- Potential cytotoxicity of trace elements and polycyclic aromatic hydrocarbons bounded to particulate matter: a review on in vitro studies on human lung epithelial cells.Environmental science and pollution research international · 2021Review
- Organ-on-a-Chip: Opportunities for Assessing the Toxicity of Particulate Matter.Frontiers in bioengineering and biotechnology · 2020Review
- The impact of polar fraction of the fine particulate matter on redox responses in different rat tissues.Environmental science and pollution research international · 2019Article
- Effects of Different Components of PMInternational journal of environmental research and public health · 2019Article
- The pollution characteristics of PMEnvironmental geochemistry and health · 2018Article
- Selective ATP-Binding Cassette Subfamily C Gene Expression and Proinflammatory Mediators Released by BEAS-2B after PMMediators of inflammation · 2017Article
- Serum cytokine levels related to exposure to volatile organic compounds and PMInternational journal of chronic obstructive pulmonary disease · 2017Article
- Effects of Size-Fractionated Particulate Matter on Cellular Oxidant Radical Generation in Human Bronchial Epithelial BEAS-2B Cells.International journal of environmental research and public health · 2016Article
- Article
- Epigallocatechin-3-gallate protects HUVECs from PM2.5-induced oxidative stress injury by activating critical antioxidant pathways.Molecules (Basel, Switzerland) · 2015Article
- Linking Endotoxins, African Dust PM10 and Asthma in an Urban and Rural Environment of Puerto Rico.Mediators of inflammation · 2015Article
- Particle pollution in Rio de Janeiro, Brazil: increase and decrease of pro-inflammatory cytokines IL-6 and IL-8 in human lung cells.Environmental pollution (Barking, Essex : 1987) · 2014Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Fine particulate air pollutants, mainly their organic fraction, have been demonstrated to be associated with cardiovascular and respiratory health problems. Puerto Rico has been reported to have the highest prevalence of pulmonary diseases (e.g., asthma) in the United States. The aim of this study was to assess, for the first time, the immunological response of human bronchial epithelial cells (BEAS-2B) to organic extracts isolated from airborne particulate matter (PM(2.5)) in Puerto Rico. Organic extracts from PM(2.5) collected throughout an 8-month period (2000-2001) were pooled (composite) in order to perform chemical analysis and biological activity testing. BEAS-2B cells were exposed to PM(2.5) organic extract to assess cytotoxicity, levels of cytokines and relative gene expression of MHC-II, hPXR and CYP3A5. Our findings show that organic PM(2.5) consist of toxic as well as bioactive components that can regulate the secretion of cytokines in BEAS-2B, which could modulate inflammatory response in the lung. Trace element analyses confirmed the presence of metals in organic extracts highlighting the relative high abundance of Cu and Zn in polar organic extracts. Polar organic extracts exhibited dose-dependant toxicity and were found to significantly induce the release of interleukin 6 (IL-6), IL-1beta and IL-7 while significantly inhibiting the secretion of IL-8, G-CSF and MCP-1. Moreover, MHC-II transcriptional activity was up-regulated after 24 h of exposure, whereas PXR and CYP3A5 were down-regulated. This research provides a new insight into the effects of PM(2.5) organic fractions on specific effectors and their possible role in the development of respiratory inflammatory diseases in Puerto Rico.
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