ArticleJournal of structural biology2010
Cryo-electron tomography of microtubule-kinesin motor complexes.
Article in Journal of structural biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
- Subcellular reorganization upon phage infection reveals stepwise assembly of viral particles from membrane-associated precursors.Nature communications · 2026Article
- Structural switching of tubulin in the microtubule lattice.Biochemical Society transactions · 2025Review
- At the Nexus between Cytoskeleton and Vacuole: How Plant Cytoskeletons Govern the Dynamics of Large Vacuoles.International journal of molecular sciences · 2023Review
- Small molecule allosteric uncoupling of microtubule depolymerase activity from motility in human Kinesin-5 during mitotic spindle assembly.Scientific reports · 2019Article
- The yeast kinesin-5 Cin8 interacts with the microtubule in a noncanonical manner.The Journal of biological chemistry · 2017Article
- A detailed look at the cytoskeletal architecture of the Giardia lamblia ventral disc.Journal of structural biology · 2016Article
- Electron Tomography: A Three-Dimensional Analytic Tool for Hard and Soft Materials Research.Advanced materials (Deerfield Beach, Fla.) · 2015Review
- High-resolution cryo-electron microscopy on macromolecular complexes and cell organelles.Protoplasma · 2014Review
- Common mechanistic themes for the powerstroke of kinesin-14 motors.Journal of structural biology · 2013Article
- The asymmetric structure of an icosahedral virus bound to its receptor suggests a mechanism for genome release.Structure (London, England : 1993) · 2013Article
- Functional asymmetry in kinesin and dynein dimers.Biology of the cell · 2013Review
- Article
- The ATPase pathway that drives the kinesin-14 Kar3Vik1 powerstroke.The Journal of biological chemistry · 2012Article
- Kar3Vik1, a member of the kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern.The Journal of cell biology · 2012Article
- Cryo-electron tomography and 3-D analysis of the intact flagellum in Trypanosoma brucei.Journal of structural biology · 2012Article
- A detailed, hierarchical study of Giardia lamblia's ventral disc reveals novel microtubule-associated protein complexes.PloS one · 2012Article
- Limiting factors in single particle cryo electron tomography.Computational and structural biotechnology journal · 2012Review
- Microtubules in bacteria: Ancient tubulins build a five-protofilament homolog of the eukaryotic cytoskeleton.PLoS biology · 2011Article
- Clustering and variance maps for cryo-electron tomography using wedge-masked differences.Journal of structural biology · 2011Article
- Cryo-electron tomography for structural characterization of macromolecular complexes.Current protocols in protein science · 2011Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Microtubules complexed with molecular motors of the kinesin family or non-motor microtubule associated proteins (MAPs) such as tau or EB1 have been the subject of cryo-electron microcopy based 3-D studies for several years. Most of these studies that targeted complexes with intact microtubules have been carried out by helical 3-D reconstruction, while few were analyzed by single particle approaches or from 2-D crystalline arrays. Helical reconstruction of microtubule-MAP or motor complexes has been extremely successful but by definition, all helical 3-D reconstruction attempts require perfectly helical assemblies, which presents a serious limitation and confines the attempts to 15- or 16-protofilament microtubules, microtubule configurations that are very rare in nature. The rise of cryo-electron tomography within the last few years has now opened a new avenue towards solving 3-D structures of microtubule-MAP complexes that do not form helical assemblies, most importantly for the subject here, all microtubules that exhibit a lattice seam. In addition, not all motor domains or MAPs decorate the microtubule surface regularly enough to match the underlying microtubule lattice, or they adopt conformations that deviate from helical symmetry. Here we demonstrate the power and limitation of cryo-electron tomography using two kinesin motor domains, the monomeric Eg5 motor domain, and the heterodimeric Kar3Vik1 motor. We show here that tomography does not exclude the possibility of post-tomographic averaging when identical sub-volumes can be extracted from tomograms and in both cases we were able to reconstruct 3-D maps of conformations that are not possible to obtain using helical or other averaging-based methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.