ReviewQuarterly reviews of biophysics2009
Single-particle reconstruction of biological macromolecules in electron microscopy--30 years.
Review in Quarterly reviews of biophysics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed.
- Engineered Cas9 complexes establish an experimentally grounded benchmark for heterogeneous cryoEM reconstruction methods.bioRxiv : the preprint server for biology · 2026Article
- Article
- Seeing the Supracolloidal Assemblies in 3D: Unraveling High-Resolution Structures Using Electron Tomography.ACS materials Au · 2024Review
- Detecting continuous structural heterogeneity in single-molecule localization microscopy data.Scientific reports · 2023Article
- Electrospray ion beam deposition plus low-energy electron holography as a tool for imaging individual biomolecules.Essays in biochemistry · 2023Article
- Article
- Mapping molecular complexes with super-resolution microscopy and single-particle analysis.Open biology · 2022Review
- Cryo-electron tomography related radiation-damage parameters for individual-molecule 3D structure determination.Frontiers in chemistry · 2022Review
- Single-Run Catalysis and Kinetic Control of Human Telomerase Holoenzyme.Advances in experimental medicine and biology · 2022Review
- Low-energy electron holography imaging of conformational variability of single-antibody molecules from electrospray ion beam deposition.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Detecting structural heterogeneity in single-molecule localization microscopy data.Nature communications · 2021Article
- Nanoscale imaging using differential expansion microscopy.Histochemistry and cell biology · 2020Article
- A simulated annealing approach for resolution guided homogeneous cryo-electron microscopy image selection.Quantitative biology (Beijing, China) · 2020Article
- Single-Molecule 3D Images of "Hole-Hole" IgG1 Homodimers by Individual-Particle Electron Tomography.Scientific reports · 2019Article
- Deep Learning for Validating and Estimating Resolution of Cryo-Electron Microscopy Density MapsMolecules (Basel, Switzerland) · 2019Article
- Using Spectral Representation to Classify Proteins' Conformational States.International journal of molecular sciences · 2018Article
- Analytical Approaches to Improve Accuracy in Solving the Protein Topology Problem.Molecules (Basel, Switzerland) · 2018Article
- Article
- Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2018Article
- The human mitochondrial Hsp60 in the APO conformation forms a stable tetradecameric complex.Cell cycle (Georgetown, Tex.) · 2017Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author.
Funding
Abstract
This essay gives the autho's personal account on the development of concepts underlying single-particle reconstruction, a technique in electron microscopy of macromolecular assemblies with a remarkable record of achievements as of late. The ribosome proved to be an ideal testing ground for the development of specimen preparation methods, cryo-EM techniques, and algorithms, with discoveries along the way as a rich reward. Increasingly, cryo-EM and single-particle reconstruction, in combination with classification techniques, is revealing dynamic information on functional molecular machines uninhibited by molecular contacts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.