ArticleBMC genomics2009
Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 111 citations in OpenAlex.
- Time-resolved transcriptomics reveals mechanisms of acute and sustained low-pH adaptation inSynthetic and systems biotechnology · 2026Article
- A Comprehensive Review on the Pharmacological Activities and Biosynthetic Strategies of Protocatechuic Acid.Life (Basel, Switzerland) · 2026Review
- Engineering of Corynebacterium glutamicum for biosynthesis of the pharmaceutically active N-acetyltyramine: establishing and optimizing de novo production.Journal of biological engineering · 2026Article
- Engineering of Corynebacterium glutamicum for the enhanced production of optically pure (meso)-2,3-butanediol.Bioresources and bioprocessing · 2026Article
- Acetate-containing supernatants from industrial off-gas cultivation enabling high-value product formation with established and emerging production organisms.Biotechnology for biofuels and bioproducts · 2026Article
- Regulatory mechanism of ferrous ion in enhancing the biosynthesis of L-homoserine, L-threonine, and L-isoleucine in Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2025Article
- Iron-based microbial interactions: the role of iron metabolism in the cheese ecosystem.Journal of bacteriology · 2025Review
- Response surface-based media optimization for astaxanthin production inFrontiers in bioengineering and biotechnology · 2025Article
- Advances in stress-tolerance elements for microbial cell factories.Synthetic and systems biotechnology · 2024Review
- Efficient biosynthesis of creatine by whole-cell catalysis from guanidinoacetic acid inSynthetic and systems biotechnology · 2024Article
- Utilization of orange peel waste for sustainable amino acid production byFrontiers in bioengineering and biotechnology · 2024Article
- Transcriptome Analysis Reveals the Regulation ofInternational journal of molecular sciences · 2023Article
- Article
- Improving growth properties of Corynebacterium glutamicum by implementing an iron-responsive protocatechuate biosynthesis.Microbial biotechnology · 2023Article
- Efficient secretory production of proline/alanine/serine (PAS) biopolymers in Corynebacterium glutamicum yielding a monodisperse biological alternative to polyethylene glycol (PEG).Microbial cell factories · 2022Article
- Production of γ-Aminobutyrate (GABA) in RecombinantACS omega · 2022Article
- Article
- Growth Response and Recovery ofFrontiers in microbiology · 2021Article
- A tunable L-arabinose-inducible expression plasmid for the acetic acid bacterium Gluconobacter oxydans.Applied microbiology and biotechnology · 2020Article
- The Iron Deficiency Response of Corynebacterium glutamicum and a Link to Thiamine Biosynthesis.Applied and environmental microbiology · 2020Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe maintenance of internal pH in bacterial cells is challenged by natural stress conditions, during host infection or in biotechnological production processes. Comprehensive transcriptomic and proteomic analyses has been conducted in several bacterial model systems, yet questions remain as to the mechanisms of pH homeostasis.
resultsHere we present the comprehensive analysis of pH homeostasis in C. glutamicum, a bacterium of industrial importance. At pH values between 6 and 9 effective maintenance of the internal pH at 7.5 +/- 0.5 pH units was found. By DNA microarray analyses differential mRNA patterns were identified. The expression profiles were validated and extended by 1D-LC-ESI-MS/MS based quantification of soluble and membrane proteins. Regulators involved were identified and thereby participation of numerous signaling modules in pH response was found. The functional analysis revealed for the first time the occurrence of oxidative stress in C. glutamicum cells at neutral and low pH conditions accompanied by activation of the iron starvation response. Intracellular metabolite pool analysis unraveled inhibition of the TCA and other pathways at low pH. Methionine and cysteine synthesis were found to be activated via the McbR regulator, cysteine accumulation was observed and addition of cysteine was shown to be toxic under acidic conditions.
conclusionsNovel limitations for C. glutamicum at non-optimal pH values were identified by a comprehensive analysis on the level of the transcriptome, proteome, and metabolome indicating a functional link between pH acclimatization, oxidative stress, iron homeostasis, and metabolic alterations. The results offer new insights into bacterial stress physiology and new starting points for bacterial strain design or pathogen defense.
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