ArticleAnalytical chemistry2010
Nonlinear optical imaging of integral membrane protein crystals in lipidic mesophases.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Biological and Biomedical Applications of Optical Photothermal Infrared Spectroscopy (O-PTIR).Applied spectroscopy · 2026Review
- Depth-of-field extension in optical imaging for rapid crystal screening.Acta crystallographica. Section D, Structural biology · 2021Article
- Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures.Journal of applied crystallography · 2020Article
- Visualization of protein crystals by high-energy phase-contrast X-ray imaging.Acta crystallographica. Section D, Structural biology · 2019Article
- A low-cost method for visible fluorescence imaging.Acta crystallographica. Section F, Structural biology communications · 2017Article
- Dynamic X-ray diffraction sampling for protein crystal positioning.Journal of synchrotron radiation · 2017Article
- Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography.Journal of visualized experiments : JoVE · 2016Article
- Serial femtosecond crystallography datasets from G protein-coupled receptors.Scientific data · 2016Article
- Current advances in synchrotron radiation instrumentation for MX experiments.Archives of biochemistry and biophysics · 2016Review
- Second harmonic generation correlation spectroscopy for characterizing translationally diffusing protein nanocrystals.Acta crystallographica. Section D, Structural biology · 2016Article
- Guiding synchrotron X-ray diffraction by multimodal video-rate protein crystal imaging.Journal of synchrotron radiation · 2016Article
- Fast two-dimensional grid and transmission X-ray microscopy scanning methods for visualizing and characterizing protein crystals.Journal of applied crystallography · 2016Article
- Intercalating dyes for enhanced contrast in second-harmonic generation imaging of protein crystals.Acta crystallographica. Section D, Biological crystallography · 2015Article
- A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.Acta crystallographica. Section F, Structural biology communications · 2015Review
- X-ray Transparent Microfluidic Chip for Mesophase-Based Crystallization of Membrane Proteins and On-Chip Structure Determination.Crystal growth & design · 2014Article
- Article
- Identifying, studying and making good use of macromolecular crystals.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Femtosecond crystallography of membrane proteins in the lipidic cubic phase.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography.Nature communications · 2014Article
- Membrane protein structure determination - the next generation.Biochimica et biophysica acta · 2014Review
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4 authors.
Funding
Abstract
Second-order nonlinear optical imaging of chiral crystals (SONICC) is explored for selective detection of integral membrane protein crystals grown in opaque and turbid environments. High turbidity is a hallmark of membrane protein crystallization due to the extensive use of detergent and/or lipids that often form various mesophases. Detection of crystals in such media by conventional optical methods (e.g., intrinsic UV fluorescence, birefringence, bright-field image analysis, etc.) is often complicated by optical scattering and by the small sizes of the crystals that routinely form. SONICC is shown to be well-suited for this application, by nature of its compatibility with imaging in scattering media and its high selectivity for protein crystals. Bright second harmonic generation (SHG) (up to 18 million counts/s) was observed from even relatively small crystals (5 mum) with a minimal background due to the surrounding lipid mesophase ( approximately 1 thousand counts/s). The low background nature of the resulting protein crystal images permitted the use of a relatively simple, particle counting analysis for preliminary scoring. Comparisons between a particle counting analysis of SONICC images and protocols based on the human expert analysis of conventional bright-field and birefringence images were performed.
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