ArticleMarine biotechnology (New York, N.Y.)2010
Antifreeze protein in Antarctic marine diatom, Chaetoceros neogracile.
Article in Marine biotechnology (New York, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 72 citations in OpenAlex.
- Ecological responses of AntarcticFrontiers in microbiology · 2026Article
- Article
- Structural diversity of marine anti-freezing proteins, properties and potential applications: a review.Bioresources and bioprocessing · 2022Review
- Cold adaptation strategies in plants-An emerging role of epigenetics and antifreeze proteins to engineer cold resilient plants.Frontiers in genetics · 2022Review
- Experimental freezing of freshwater pennate diatoms from polar habitats.Protoplasma · 2021Article
- Prediction and analysis of antifreeze proteins.Heliyon · 2021Article
- Identification of Biomolecules Involved in the Adaptation to the Environment of Cold-Loving Microorganisms and Metabolic Pathways for Their Production.Biomolecules · 2021Review
- Article
- Molecular basis of ice-binding and cryopreservation activities of type III antifreeze proteins.Computational and structural biotechnology journal · 2021Article
- Physiological and Molecular Responses to Main Environmental Stressors of Microalgae and Bacteria in Polar Marine Environments.Microorganisms · 2020Review
- Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture.Biomolecules · 2020Review
- Heterologous Gene Expression System Using the Cold-InducibleJournal of microbiology and biotechnology · 2020Article
- Annual Cycle of Freshwater Diatoms in the High Arctic Revealed by Multiparameter Fluorescent Staining.Microbial ecology · 2020Article
- Review
- Growth suppression of ice crystal basal face in the presence of a moderate ice-binding protein does not confer hyperactivity.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Draft genome sequences of bacteria isolated from the Deschampsia antarctica phyllosphere.Extremophiles : life under extreme conditions · 2018Article
- Identification and Characterization of an Isoform Antifreeze Protein from the Antarctic Marine Diatom, Chaetoceros neogracile and Suggestion of the Core Region.Marine drugs · 2017Article
- Structure of a 1.5-MDa adhesin that binds its Antarctic bacterium to diatoms and ice.Science advances · 2017Article
- Marine Antifreeze Proteins: Structure, Function, and Application to Cryopreservation as a Potential Cryoprotectant.Marine drugs · 2017Review
- New Cysteine-Rich Ice-Binding Protein Secreted from Antarctic Microalga, Chloromonas sp.PloS one · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The antifreeze protein gene (Cn-AFP) from the Antarctic marine diatom, Chaetoceros neogracile was cloned and characterized. The full-length Cn-AFP cDNA contained an open reading frame of 849 bp and the deduced 282 amino acid peptide chain encodes a 29.2 kDa protein, which includes a signal peptide of 30 amino acids at the N terminus. Both the Cn-AFP coding region with and without the signal sequence were cloned and expressed in Escherichia coli. Recombinant Cn-AFPs were shown to display antifreeze activities based on measuring the thermal hysteresis and modified morphology of single ice crystals. Recombinant mature Cn-AFP showed 16-fold higher thermal hysteresis activity than that of pre-mature Cn-AFP at the same concentration. The ice crystal shape changed to an elongated hexagonal shape in the presence of the recombinant mature Cn-AFP, while single ice crystal showed a circular disk shape in absence of Cn-AFP. Northern analysis demonstrated a dramatic accumulation of Cn-AFP transcripts when the cells were subjected to freezing stress. This rapid response to freeze stress, and the antifreeze activity of recombinant Cn-AFPs, indicates that Cn-AFP plays an important role in low temperature adaptation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.