ArticleLab on a chip2010
Electrically evoking and electrochemically resolving quantal release on a microchip.
Article in Lab on a chip, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- Microfluidic platforms for single neuron analysis.Materials today. Bio · 2022Review
- Instrumented Microphysiological Systems for Real-Time Measurement and Manipulation of Cellular Electrochemical Processes.iScience · 2019Review
- Electrochemical measurement of quantal exocytosis using microchips.Pflugers Archiv : European journal of physiology · 2018Review
- The Roles of Biofilm Conductivity and Donor Substrate Kinetics in a Mixed-Culture Biofilm Anode.Environmental science & technology · 2016Article
- All-carbon multi-electrode array for real-time in vitro measurements of oxidizable neurotransmitters.Scientific reports · 2016Article
- Two approaches for addressing electrochemical electrode arrays with reduced external connections.Analytical methods : advancing methods and applications · 2015Article
- Carbon fiber ultramicrodic electrode electrodeposited with over-oxidized polypyrrole for amperometric detection of vesicular exocytosis from pheochromocytoma cell.Sensors (Basel, Switzerland) · 2015Article
- Development and characterization of a diamond-insulated graphitic multi electrode array realized with ion beam lithography.Sensors (Basel, Switzerland) · 2014Article
- Heterogeneous distribution of exocytotic microdomains in adrenal chromaffin cells resolved by high-density diamond ultra-microelectrode arrays.The Journal of physiology · 2014Article
- Spatial resolution of single-cell exocytosis by microwell-based individually addressable thin film ultramicroelectrode arrays.Analytical chemistry · 2014Article
- A microfluidic platform for chemical stimulation and real time analysis of catecholamine secretion from neuroendocrine cells.Lab on a chip · 2013Article
- Electroporation followed by electrochemical measurement of quantal transmitter release from single cells using a patterned microelectrode.Lab on a chip · 2013Article
- Fabrication of two-layer poly(dimethyl siloxane) devices for hydrodynamic cell trapping and exocytosis measurement with integrated indium tin oxide microelectrodes arrays.Biomedical microdevices · 2013Article
- Determination of mammalian cell counts, cell size and cell health using the Moxi Z mini automated cell counter.Journal of visualized experiments : JoVE · 2012Article
- Quantification of noise sources for amperometric measurement of quantal exocytosis using microelectrodes.The Analyst · 2012Article
- Microwell device for targeting single cells to electrochemical microelectrodes for high-throughput amperometric detection of quantal exocytosis.Analytical chemistry · 2011Article
- Automated targeting of cells to electrochemical electrodes using a surface chemistry approach for the measurement of quantal exocytosis.ACS chemical neuroscience · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A microchip was applied to electrically depolarize rat pheochromocytoma (PC12) cells and to simultaneously detect exocytotic catecholamine release amperometrically. Results demonstrate exocytosis elicited by flowing cells through an electric field generated by a potentiostat circuit in a microchannel, as well as exocytosis triggered by application of an extracellular voltage pulse across. Electrical finite element model (FEM) analysis illustrated that larger cells experienced greater depolarizing excitation from the extracellular electric fields due to the smaller shunt path and higher resistance to current flow in the channel around the cell. Consistent with these simulations, data recorded from cell clusters and large cells exhibited increased release rates relative to data from the smaller cells. Overall, the system was capable of resolving single vesicle quantal release, in the zeptomole range, as well as the kinetics associated with the vesicle fusion process. Analysis of spike population statistics suggested detection of catecholamines from multiple release sites around the cells. The potential for such a device to be used in flow cytometry to evoke and detect exocytosis was demonstrated.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.