ArticleThe Journal of biological chemistry2010
Modifications of protein environment of the [2Fe-2S] cluster of the bc1 complex: effects on the biophysical properties of the rieske iron-sulfur protein and on the kinetics of the complex.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- The cytochrome bPhotosynthesis research · 2024Review
- Modeling the Energy Landscape of Side Reactions in the Cytochrome bcFrontiers in chemistry · 2021Article
- Dissecting the pattern of proton release from partial process involved in ubihydroquinone oxidation in the Q-cycle.Biochimica et biophysica acta. Bioenergetics · 2018Article
- Quinol oxidase encoded by cyoABCD in Rhizobium etli CFN42 is regulated by ActSR and is crucial for growth at low pH or low iron conditions.Microbiology (Reading, England) · 2015Article
- Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cells.Free radical biology & medicine · 2015Article
- Communication: Microsecond dynamics of the protein and water affect electron transfer in a bacterial bc(1) complex.The Journal of chemical physics · 2015Article
- Identification of ubiquinol binding motifs at the Qo-site of the cytochrome bc1 complex.The journal of physical chemistry. B · 2015Article
- Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.Chemical reviews · 2014Review
- Electron transfer and proton-coupled electron transfer reactivity and self-exchange of synthetic [2Fe-2S] complexes: models for Rieske and mitoNEET clusters.Inorganic chemistry · 2014Article
- Fast proton-coupled electron transfer observed for a high-fidelity structural and functional [2Fe-2S] Rieske model.Journal of the American Chemical Society · 2014Article
- pH-dependent regulation of electron transport and ATP synthesis in chloroplasts.Photosynthesis research · 2013Review
- Role of the -PEWY-glutamate in catalysis at the Q(o)-site of the Cyt bc(1) complex.Biochimica et biophysica acta · 2013Article
- The respiratory arsenite oxidase: structure and the role of residues surrounding the rieske cluster.PloS one · 2013Article
- Protonation and concerted proton-electron transfer reactivity of a bis-benzimidazolate ligated [2Fe-2S] model for Rieske clusters.Journal of the American Chemical Society · 2012Article
- Article
- Impaired OXPHOS complex III in breast cancer.PloS one · 2011Article
- NMR investigations of the Rieske protein from Thermus thermophilus support a coupled proton and electron transfer mechanism.Journal of the American Chemical Society · 2010Article
- The mechanism of ubihydroquinone oxidation at the Qo-site of the cytochrome bc1 complex.Biochimica et biophysica actaReview
- Design and use of photoactive ruthenium complexes to study electron transfer within cytochrome bc1 and from cytochrome bc1 to cytochrome c.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The rate-determining step in the overall turnover of the bc(1) complex is electron transfer from ubiquinol to the Rieske iron-sulfur protein (ISP) at the Q(o)-site. Structures of the ISP from Rhodobacter sphaeroides show that serine 154 and tyrosine 156 form H-bonds to S-1 of the [2Fe-2S] cluster and to the sulfur atom of the cysteine liganding Fe-1 of the cluster, respectively. These are responsible in part for the high potential (E(m)(,7) approximately 300 mV) and low pK(a) (7.6) of the ISP, which determine the overall reaction rate of the bc(1) complex. We have made site-directed mutations at these residues, measured thermodynamic properties using protein film voltammetry to evaluate the E(m) and pK(a) values of ISPs, explored the local proton environment through two-dimensional electron spin echo envelope modulation, and characterized function in strains S154T, S154C, S154A, Y156F, and Y156W. Alterations in reaction rate were investigated under conditions in which concentration of one substrate (ubiquinol or ISP(ox)) was saturating and the other was varied, allowing calculation of kinetic terms and relative affinities. These studies confirm that H-bonds to the cluster or its ligands are important determinants of the electrochemical characteristics of the ISP, likely through electron affinity of the interacting atom and the geometry of the H-bonding neighborhood. The calculated parameters were used in a detailed Marcus-Brønsted analysis of the dependence of rate on driving force and pH. The proton-first-then-electron model proposed accounts naturally for the effects of mutation on the overall reaction.
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