ArticleThe Plant cell2009
Arabidopsis formin3 directs the formation of actin cables and polarized growth in pollen tubes.
Article in The Plant cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 137 citations in OpenAlex.
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- Genome-wide characterization and expression profiling of FIMBRIN gene family members in response to abiotic stress in Medicago sativa.BMC plant biology · 2025Article
- Establishing cell polarity in plants: the role of cytoskeletal structures and regulatory pathways.Frontiers in cell and developmental biology · 2025Review
- The fate and function of theFrontiers in plant science · 2025Article
- AtFH5 recruits and transports the arabinogalactan protein AGP23 to maintain the tip growth of pollen tube.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Transmembrane formins as active cargoes of membrane trafficking.Journal of experimental botany · 2024Review
- From gametes to zygote: Mechanistic advances and emerging possibilities in plant reproduction.Plant physiology · 2024Review
- Genome-Wide Studies ofPlants (Basel, Switzerland) · 2024Article
- Genome-Wide Identification and Expression Analysis of thePlants (Basel, Switzerland) · 2023Article
- Formin protein DRT1 affects gross morphology and chloroplast relocation in rice.Plant physiology · 2023Article
- Actin-bundling protein fimbrin regulates pathogenicity via organizing F-actin dynamics during appressorium development in Colletotrichum gloeosporioides.Molecular plant pathology · 2022Article
- O-glycosylation of the extracellular domain of pollen class I formins modulates their plasma membrane mobility.Journal of experimental botany · 2022Article
- The bHLH1-DTX35/DFR module regulates pollen fertility by promoting flavonoid biosynthesis inHorticulture research · 2022Article
- Article
- Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra.Horticulture research · 2021Article
- Analysis of formin functions during cytokinesis using specific inhibitor SMIFH2.Plant physiology · 2021Article
- The quest for the central players governing pollen tube growth and guidance.Plant physiology · 2021Review
- TheFrontiers in plant science · 2021Article
- An Update on the Role of the Actin Cytoskeleton in Plasmodesmata: A Focus on Formins.Frontiers in plant science · 2021Review
- Exocyst mutants suppress pollen tube growth and cell wall structural defects of hydroxyproline O-arabinosyltransferase mutants.The Plant journal : for cell and molecular biology · 2020Article
13 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytoplasmic actin cables are the most prominent actin structures in plant cells, but the molecular mechanism underlying their formation is unknown. The function of these actin cables, which are proposed to modulate cytoplasmic streaming and intracellular movement of many organelles in plants, has not been studied by genetic means. Here, we show that Arabidopsis thaliana formin3 (AFH3) is an actin nucleation factor responsible for the formation of longitudinal actin cables in pollen tubes. The Arabidopsis AFH3 gene encodes a 785-amino acid polypeptide, which contains a formin homology 1 (FH1) and a FH2 domain. In vitro analysis revealed that the AFH3 FH1FH2 domains interact with the barbed end of actin filaments and have actin nucleation activity in the presence of G-actin or G actin-profilin. Overexpression of AFH3 in tobacco (Nicotiana tabacum) pollen tubes induced excessive actin cables, which extended into the tubes' apices. Specific downregulation of AFH3 eliminated actin cables in Arabidopsis pollen tubes and reduced the level of actin polymers in pollen grains. This led to the disruption of the reverse fountain streaming pattern in pollen tubes, confirming a role for actin cables in the regulation of cytoplasmic streaming. Furthermore, these tubes became wide and short and swelled at their tips, suggesting that actin cables may regulate growth polarity in pollen tubes. Thus, AFH3 regulates the formation of actin cables, which are important for cytoplasmic streaming and polarized growth in pollen tubes.
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