ArticleDevelopment (Cambridge, England)2010
Distinguishing direct from indirect roles for bicoid mRNA localization factors.
Article in Development (Cambridge, England), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Dynamics of bicoid mRNA localisation and translation dictate morphogen gradient formation.The EMBO journal · 2026Article
- Article
- Review
- Cortical movement of Bicoid in early Drosophila embryos is actin- and microtubule-dependent and disagrees with the SDD diffusion model.PloS one · 2017Article
- Article
- Fixed and live visualization of RNAs in Drosophila oocytes and embryos.Methods (San Diego, Calif.) · 2016Review
- The pros and cons of common actin labeling tools for visualizing actin dynamics during Drosophila oogenesis.Developmental biology · 2014Article
- The multifunctional Staufen proteins: conserved roles from neurogenesis to synaptic plasticity.Trends in neurosciences · 2014Review
- Subcellular mRNA localisation at a glance.Journal of cell science · 2014Review
- mRNA localization in the Drosophila germline.RNA biology · 2014Review
- Structural features of LC8-induced self-association of swallow.Biochemistry · 2013Article
- mRNA localization and translational control in Drosophila oogenesis.Cold Spring Harbor perspectives in biology · 2012Review
- Control of cytoplasmic mRNA localization.Cellular and molecular life sciences : CMLS · 2012Review
- Remodelling of cortical actin where lytic granules dock at natural killer cell immune synapses revealed by super-resolution microscopy.PLoS biology · 2011Article
- mRNA localization: assembly of transport complexes and their incorporation into particles.Current opinion in genetics & development · 2011Review
- Anterior-posterior axis specification in Drosophila oocytes: identification of novel bicoid and oskar mRNA localization factors.Genetics · 2011Article
- Molecular motors: directing traffic during RNA localization.Critical reviews in biochemistry and molecular biology · 2011Review
- The formation of the Bicoid morphogen gradient requires protein movement from anteriorly localized mRNA.PLoS biology · 2011Article
- Making the message clear: visualizing mRNA localization.Trends in cell biology · 2010Review
- ISH-IEM: a sensitive method to detect endogenous mRNAs at the ultrastructural level.Nature protocols · 2010Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Localization of bicoid mRNA to the anterior of the Drosophila oocyte is essential for patterning the anteroposterior body axis in the early embryo. bicoid mRNA localizes in a complex multistep process involving transacting factors, molecular motors and cytoskeletal components that remodel extensively during the lifetime of the mRNA. Genetic requirements for several localization factors, including Swallow and Staufen, are well established, but the precise roles of these factors and their relationship to bicoid mRNA transport particles remains unresolved. Here we use live cell imaging, super-resolution microscopy in fixed cells and immunoelectron microscopy on ultrathin frozen sections to study the distribution of Swallow, Staufen, actin and dynein relative to bicoid mRNA during late oogenesis. We show that Swallow and bicoid mRNA are transported independently and are not colocalized at their final destination. Furthermore, Swallow is not required for bicoid transport. Instead, Swallow localizes to the oocyte plasma membrane, in close proximity to actin filaments, and we present evidence that Swallow functions during the late phase of bicoid localization by regulating the actin cytoskeleton. In contrast, Staufen, dynein and bicoid mRNA form nonmembranous, electron dense particles at the oocyte anterior. Our results exclude a role for Swallow in linking bicoid mRNA to the dynein motor. Instead we propose a model for bicoid mRNA localization in which Swallow is transported independently by dynein and contributes indirectly to bicoid mRNA localization by organizing the cytoskeleton, whereas Staufen plays a direct role in dynein-dependent bicoid mRNA transport.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.