ArticlePlant physiology2010
Generation of backbone-free, low transgene copy plants by launching T-DNA from the Agrobacterium chromosome.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Overcoming breeding barriers with genome editing in autopolyploid crops.BMC plant biology · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- The evolving landscape of precise DNA insertion in plants.Nature communications · 2025Review
- Binary vector origin predictably determinesbioRxiv : the preprint server for biology · 2025Article
- T-DNA orientation, distance between two T-DNAs, and the transformation target cells significantly impact vector backbone integration and efficiency of generating marker-free transgenic plants in a co-transformation system.The Plant journal : for cell and molecular biology · 2025Article
- Binary vector copy number engineering improves Agrobacterium-mediated transformation.Nature biotechnology · 2025Article
- Co-transformation using T-DNA genes from Agrobacterium strain 82.139 enhances regeneration of transgenic shoots in Populus.Plant biotechnology journal · 2025Article
- A review of factors affecting the success of geminivirus infectious clones.Plant cell reports · 2025Review
- Enhancing biolistic plant transformation and genome editing with a flow guiding barrel.Nature communications · 2025Article
- Engineering Agrobacterium for improved plant transformation.The Plant journal : for cell and molecular biology · 2025Review
- Genomic consequences associated with Agrobacterium-mediated transformation of plants.The Plant journal : for cell and molecular biology · 2024Review
- Prime editing: Mechanism insight and recent applications in plants.Plant biotechnology journal · 2024Review
- Highly Efficient CRISPR/Cas9 Mediated Gene Editing inPlants (Basel, Switzerland) · 2023Article
- Intracellular bottlenecking permits no more than three tomato yellow leaf curl virus genomes to initiate replication in a single cell.PLoS pathogens · 2023Article
- Review
- Ectopic expression ofBiotechnology reports (Amsterdam, Netherlands) · 2022Article
- Overexpression of SoybeanInternational journal of molecular sciences · 2022Article
- Studies on Pure MlbFrontiers in plant science · 2022Article
- Molecular characterization for food safety assessment of a genetically modified late blight resistant potato: an unusual case.Transgenic research · 2021Article
- Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In both applied and basic research, Agrobacterium-mediated transformation is commonly used to introduce genes into plants. We investigated the effect of three Agrobacterium tumefaciens strains and five transferred (T)-DNA origins of replication on transformation frequency, transgene copy number, and the frequency of integration of non-T-DNA portions of the T-DNA-containing vector (backbone) into the genome of Arabidopsis (Arabidopsis thaliana) and maize (Zea mays). Launching T-DNA from the picA locus of the Agrobacterium chromosome increases the frequency of single transgene integration events and almost eliminates the presence of vector backbone sequences in transgenic plants. Along with novel Agrobacterium strains we have developed, our findings are useful for improving the quality of T-DNA integration events.
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Registered trials
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