ArticleApplied and environmental microbiology2010
Improved electrotransformation and decreased antibiotic resistance of the cystic fibrosis pathogen Burkholderia cenocepacia strain J2315.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- A High-Throughput Microtiter Plate Screening Assay to Quantify and Differentiate Species in Dual-Species Biofilms.Microorganisms · 2023Article
- Phenazines and toxoflavin act as interspecies modulators of resilience to diverse antibiotics.Molecular microbiology · 2022Article
- Biosynthesis of Ditropolonyl Sulfide, an Antibacterial Compound Produced by Burkholderia cepacia Complex Strain R-12632.Applied and environmental microbiology · 2021Article
- Conservation of Resistance-Nodulation-Cell Division Efflux Pump-Mediated Antibiotic Resistance in Burkholderia cepacia Complex and Burkholderia pseudomallei Complex Species.Antimicrobial agents and chemotherapy · 2021Article
- Generation of Genetic Tools for Gauging Multiple-Gene Expression at the Single-Cell Level.Applied and environmental microbiology · 2021Article
- Versatile nourseothricin and streptomycin/spectinomycin resistance gene cassettes and their use in chromosome integration vectors.Journal of microbiological methods · 2016Article
- Orderly Replication and Segregation of the Four Replicons of Burkholderia cenocepacia J2315.PLoS genetics · 2016Article
- Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDIS.Frontiers in microbiology · 2016Article
- Single amino acid substitution in homogentisate 1,2-dioxygenase is responsible for pigmentation in a subset of Burkholderia cepacia complex isolates.Environmental microbiology reports · 2015Article
- Efflux pump-mediated drug resistance in Burkholderia.Frontiers in microbiology · 2015Article
- Investigation of aminoglycoside resistance inducing conditions and a putative AmrAB-OprM efflux system in Burkholderia vietnamiensis.Annals of clinical microbiology and antimicrobials · 2014Article
- Article
- Centromere binding and evolution of chromosomal partition systems in the Burkholderiales.Journal of bacteriology · 2012Article
- Identification of hopanoid biosynthesis genes involved in polymyxin resistance in Burkholderia multivorans.Antimicrobial agents and chemotherapy · 2012Article
- Characterization of the poly-β-1,6-N-acetylglucosamine polysaccharide component of Burkholderia biofilms.Applied and environmental microbiology · 2011Article
- Exploring the metabolic network of the epidemic pathogen Burkholderia cenocepacia J2315 via genome-scale reconstruction.BMC systems biology · 2011Article
- In vitro susceptibility of Burkholderia vietnamiensis to aminoglycosides.Antimicrobial agents and chemotherapy · 2011Article
- Burkholderia glumae: next major pathogen of rice?Molecular plant pathology · 2011Review
- Burkholderia cepacia Complex: Emerging Multihost Pathogens Equipped with a Wide Range of Virulence Factors and Determinants.International journal of microbiology · 2011Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bacterium Burkholderia cenocepacia is pathogenic for sufferers from cystic fibrosis (CF) and certain immunocompromised conditions. The B. cenocepacia strain most frequently isolated from CF patients, and which serves as the reference for CF epidemiology, is J2315. The J2315 genome is split into three chromosomes and one plasmid. The strain was sequenced several years ago, and its annotation has been released recently. This information should allow genetic experimentation with J2315, but two major impediments appear: the poor potential of J2315 to act as a recipient in transformation and conjugation and the high level of resistance it mounts to nearly all antibiotics. Here, we describe modifications to the standard electroporation procedure that allow routine transformation of J2315 by DNA. In addition, we show that deletion of an efflux pump gene and addition of spermine to the medium enhance the sensitivity of J2315 to certain commonly used antibiotics and so allow a wider range of antibiotic resistance genes to be used for selection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.