ArticleJournal of bacteriology2010
Rhizobium leguminosarum hupE encodes a nickel transporter required for hydrogenase activity.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Signal-strapping as a protein-sequence search method for the discovery of metalloproteins.Nature communications · 2025Article
- Two zinc ABC transporters contribute toFrontiers in plant science · 2025Article
- Metal nutrition and transport in the process of symbiotic nitrogen fixation.Plant communications · 2024Review
- Proteomic and Mutant Analysis of Hydrogenase Maturation Protein GeneInternational journal of molecular sciences · 2023Article
- What determines symbiotic nitrogen fixation efficiency in rhizobium: recent insights into Rhizobium leguminosarum.Archives of microbiology · 2023Review
- Signaling and Detoxification Strategies in Plant-Microbes Symbiosis under Heavy Metal Stress: A Mechanistic Understanding.Microorganisms · 2022Review
- Comparative Physiology and Genomics of Hydrogen-Producing Vibrios.Current microbiology · 2022Article
- Riboswitch regulation in cyanobacteria is independent of their habitat adaptations.Physiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
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- Trace Metal Acquisition by Marine Heterotrophic Bacterioplankton with Contrasting Trophic Strategies.Applied and environmental microbiology · 2016Article
- Transition Metal Transport in Plants and Associated Endosymbionts: Arbuscular Mycorrhizal Fungi and Rhizobia.Frontiers in plant science · 2016Review
- Genomic basis of symbiovar mimosae in Rhizobium etli.BMC genomics · 2014Article
- Fixating on metals: new insights into the role of metals in nodulation and symbiotic nitrogen fixation.Frontiers in plant science · 2014Review
- Sinorhizobium meliloti Nia is a P(1B-5)-ATPase expressed in the nodule during plant symbiosis and is involved in Ni and Fe transport.Metallomics : integrated biometal science · 2013Article
- Functional and expression analysis of the metal-inducible dmeRF system from Rhizobium leguminosarum bv. viciae.Applied and environmental microbiology · 2013Article
- Identification and characterization of RibN, a novel family of riboflavin transporters from Rhizobium leguminosarum and other proteobacteria.Journal of bacteriology · 2013Article
- Article
- Exploring the symbiotic pangenome of the nitrogen-fixing bacterium Sinorhizobium meliloti.BMC genomics · 2011Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthesis of the hydrogen uptake (Hup) system in Rhizobium leguminosarum bv. viciae requires the function of an 18-gene cluster (hupSLCDEFGHIJK-hypABFCDEX). Among them, the hupE gene encodes a protein showing six transmembrane domains for which a potential role as a nickel permease has been proposed. In this paper, we further characterize the nickel transport capacity of HupE and that of the translated product of hupE2, a hydrogenase-unlinked gene identified in the R. leguminosarum genome. HupE2 is a potential membrane protein that shows 48% amino acid sequence identity with HupE. Expression of both genes in the Escherichia coli nikABCDE mutant strain HYD723 restored hydrogenase activity and nickel transport. However, nickel transport assays revealed that HupE and HupE2 displayed different levels of nickel uptake. Site-directed mutagenesis of histidine residues in HupE revealed two motifs (HX(5)DH and FHGX[AV]HGXE) that are required for HupE functionality. An R. leguminosarum double mutant, SPF22A (hupE hupE2), exhibited reduced levels of hydrogenase activity in free-living cells, and this phenotype was complemented by nickel supplementation. Low levels of symbiotic hydrogenase activity were also observed in SPF22A bacteroid cells from lentil (Lens culinaris L.) root nodules but not in pea (Pisum sativum L.) bacteroids. Moreover, heterologous expression of the R. leguminosarum hup system in bacteroid cells of Rhizobium tropici and Mesorhizobium loti displayed reduced levels of hydrogen uptake in the absence of hupE. These data support the role of R. leguminosarum HupE as a nickel permease required for hydrogen uptake under both free-living and symbiotic conditions.
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