ArticleThe Journal of biological chemistry2010
The transcription activity of Gis1 is negatively modulated by proteasome-mediated limited proteolysis.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- TheAging · 2023Article
- The Yeast Protein Kinase Sch9 Functions as a Central Nutrient-Responsive Hub That Calibrates Metabolic and Stress-Related Responses.Journal of fungi (Basel, Switzerland) · 2023Review
- Article
- Heme promotes transcriptional and demethylase activities of Gis1, a member of the histone demethylase JMJD2/KDM4 family.Nucleic acids research · 2018Article
- Gcn5-mediated Rph1 acetylation regulates its autophagic degradation under DNA damage stress.Nucleic acids research · 2017Article
- The Yeast GSK-3 Homologue Mck1 Is a Key Controller of Quiescence Entry and Chronological Lifespan.PLoS genetics · 2015Article
- Rph1/KDM4 mediates nutrient-limitation signaling that leads to the transcriptional induction of autophagy.Current biology : CB · 2015Article
- The histone demethylase activity of Rph1 is not essential for its role in the transcriptional response to nutrient signaling.PloS one · 2014Article
- Dissociation of the H3K36 demethylase Rph1 from chromatin mediates derepression of environmental stress-response genes under genotoxic stress in Saccharomyces cerevisiae.Molecular biology of the cell · 2013Article
- Synergistic effects of TOR and proteasome pathways on the yeast transcriptome and cell growth.Open biology · 2013Article
- Gis1 and Rph1 regulate glycerol and acetate metabolism in glucose depleted yeast cells.PloS one · 2012Article
- The role of proteosome-mediated proteolysis in modulating potentially harmful transcription factor activity in Saccharomyces cerevisiae.Bioinformatics (Oxford, England) · 2011Article
- Regulation of yeast chronological life span by TORC1 via adaptive mitochondrial ROS signaling.Cell metabolism · 2011Article
- Transcriptional regulation in yeast during diauxic shift and stationary phase.Omics : a journal of integrative biology · 2010Review
- The JmjC domain of Gis1 is dispensable for transcriptional activation.FEMS yeast research · 2010Article
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2 authors.
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Abstract
The transcriptional response to environmental changes has to be prompt but appropriate. Previously, it has been shown that the Gis1 transcription factor is responsible for regulating the expression of postdiauxic shift genes in response to nutrient starvation, and this transcription regulation is dependent upon the Rim15 kinase. Here we demonstrate that the activity of Gis1 is negatively modulated by proteasome-mediated limited proteolysis. Limited degradation of Gis1 by the proteasome leads to the production of smaller variants, which have weaker transcription activities than the full-length protein. The coiled-coil domain, absent from the smaller variants, is part of the second transcription activation domain in Gis1 and is essential for both the limited proteolysis of Gis1 and its full activity. Endogenous Gis1 and its variants, regardless of their transcription capabilities, activate transcription in a Rim15-dependent manner. However, when the full-length Gis1 accumulates in cells due to overexpression or inhibition of the proteasome function, transcription activation by Gis1 is no longer solely controlled by Rim15. Together, these data strongly indicate that the function of the limited degradation is to ensure that Gis1-dependent transcription is strictly regulated by the Rim15 kinase. Furthermore, we have revealed that the kinase activity of Rim15 is essential for this regulation.
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