ArticleThe Journal of biological chemistry2010
Mg2+ dependence of 70 S ribosomal protein flexibility revealed by hydrogen/deuterium exchange and mass spectrometry.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Comparative Transcriptomic Analysis Reveals Conserved and Nutrient-Specific Responses to Nutrient Deficiencies in Quinoa.Plants (Basel, Switzerland) · 2026Article
- Magnesium-mediated stress adaptation in plants: from physio-biochemical insights to climate-resilient agriculture.Frontiers in plant science · 2026Review
- Article
- ATP:MgBiophysical journal · 2022Article
- Physiological Essence of Magnesium in Plants and Its Widespread Deficiency in the Farming System of China.Frontiers in plant science · 2022Review
- Uniting Native Capillary Electrophoresis and Multistage Ultraviolet Photodissociation Mass Spectrometry for Online Separation and Characterization ofAnalytical chemistry · 2020Article
- Establishing a EukaryoticFrontiers in bioengineering and biotechnology · 2020Article
- Arabidopsis thaliana mutants lacking cpFtsY or cpSRP54 exhibit different defects in photosystem II repair.Frontiers in plant science · 2015Article
- Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium.Journal of bacteriology · 2014Article
- Mechanism and rates of exchange of L7/L12 between ribosomes and the effects of binding EF-G.ACS chemical biology · 2012Article
- Evidence for ATP-dependent structural rearrangement of nuclease catalytic site in DNA mismatch repair endonuclease MutL.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
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Abstract
The ribosome from Escherichia coli requires a specific concentration of Mg(2+) to maintain the 70 S complex formation and allow protein synthesis, and then the structure must be stable and flexible. How does the ribosome acquire these conflicting factors at the same time? Here, we investigated the hydrogen/deuterium exchange of 52 proteins in the 70 S ribosome, which controlled stability and flexibility under various Mg(2+) concentrations, using mass spectrometry. Many proteins exhibited a sigmoidal curve for Mg(2+) concentration dependence, incorporating more deuterium at lower Mg(2+) concentration. By comparing deuterium incorporation with assembly, we have discovered a typical mechanism of complexes for acquiring both stability and flexibility at the same time. In addition, we got information of the localization of flexibility in ribosomal function by the analysis of related proteins with stalk protein, tRNA, mRNA, and nascent peptide, and demonstrate the relationship between structure, assembly, flexibility, and function of the ribosome.
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