ArticleBMC microbiology2009
Genetic tools for the investigation of Roseobacter clade bacteria.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Rational assembly of synthetic marine biofilm community with chitinase production.Research square · 2026Article
- Lifestyle-dependent evolution and CtrA-mediated lifestyle transitions shape phage resistance in marine Roseobacter.Nature communications · 2026Article
- Methionine synthesis and glycine betaine demethylation are intricately intertwined in cosmopolitan marine bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Directed assembly of biofilm communities for marine biofouling prevention.Applied and environmental microbiology · 2025Article
- The impact of tropodithietic acid on microbial physiology under varying culture complexities.mSphere · 2025Article
- Engineered Marine Biofilms for Ocean Environment Monitoring.ACS synthetic biology · 2025Article
- Blue valorization of lignin-derived monomers via reprogramming marine bacteriumApplied and environmental microbiology · 2024Article
- Modular Low-Copy-Number Plasmid Vectors for Rhodobacterales with Extended Host Range in Alphaproteobacteria.ACS synthetic biology · 2024Article
- From Natural Microbe Screening to Sustained Chitinase Activity in Exogenous Hosts.ACS synthetic biology · 2024Article
- Establishing genetic manipulation for novel strains of human gut bacteria.Microbiome research reports · 2023Review
- Trade-offs of lipid remodeling in a marine predator-prey interaction in response to phosphorus limitation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Impact of Quorum Sensing and Tropodithietic Acid Production on the Exometabolome ofFrontiers in microbiology · 2022Article
- Exploiting Aerobic Carboxydotrophic Bacteria for Industrial Biotechnology.Advances in biochemical engineering/biotechnology · 2022Article
- Translating New Synthetic Biology Advances for Biosensing Into the Earth and Environmental Sciences.Frontiers in microbiology · 2020Review
- Functional Redundancy in the Hydroxycinnamate Catabolism Pathways of the Salt Marsh Bacterium Sagittula stellata E-37.Applied and environmental microbiology · 2018Article
- Aestuarium zhoushanense gen. nov., sp. nov., Isolated from the Tidal Flat.Current microbiology · 2017Article
- Establishment of a markerless gene deletion system in Chromohalobacter salexigens DSM 3043.Extremophiles : life under extreme conditions · 2017Article
- Boosted Membrane Potential as Bioenergetic Response to Anoxia inFrontiers in microbiology · 2017Article
- Interkingdom Cross-Feeding of Ammonium from Marine Methylamine-Degrading Bacteria to the Diatom Phaeodactylum tricornutum.Applied and environmental microbiology · 2016Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Roseobacter clade represents one of the most abundant, metabolically versatile and ecologically important bacterial groups found in marine habitats. A detailed molecular investigation of the regulatory and metabolic networks of these organisms is currently limited for many strains by missing suitable genetic tools.
resultsConjugation and electroporation methods for the efficient and stable genetic transformation of selected Roseobacter clade bacteria including Dinoroseobacter shibae, Oceanibulbus indolifex, Phaeobacter gallaeciensis, Phaeobacter inhibens, Roseobacter denitrificans and Roseobacter litoralis were tested. For this purpose an antibiotic resistance screening was performed and suitable genetic markers were selected. Based on these transformation protocols stably maintained plasmids were identified. A plasmid encoded oxygen-independent fluorescent system was established using the flavin mononucleotide-based fluorescent protein FbFP. Finally, a chromosomal gene knockout strategy was successfully employed for the inactivation of the anaerobic metabolism regulatory gene dnr from D. shibae DFL12T.
conclusionA genetic toolbox for members of the Roseobacter clade was established. This provides a solid methodical basis for the detailed elucidation of gene regulatory and metabolic networks underlying the ecological success of this group of marine bacteria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.