ArticleCurrent eye research2010
Sensitivity of staurosporine-induced differentiated RGC-5 cells to homocysteine.
Article in Current eye research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Association of serum and vitreous homocysteine and uric acid concentrations with post-vitrectomy prognosis in patients with proliferative diabetic retinopathy.World journal of diabetes · 2026Article
- A Case Study from the Past: "The RGC-5 vs. the 661W Cell Line: Similarities, Differences and Contradictions-Are They Really the Same?"International journal of molecular sciences · 2023Article
- Light-Induced Retinal Ganglion Cell Damage and the Relevant Mechanisms.Cellular and molecular neurobiology · 2020Review
- Oxidative stress and mitochondrial dysfunction of retinal ganglion cells injury exposures in long-term blue light.International journal of ophthalmology · 2020Article
- Organ Cultures for Retinal Diseases.Frontiers in neuroscience · 2020Review
- Retinal Ganglion Cell Loss and Mild Vasculopathy in Methylene Tetrahydrofolate Reductase (Mthfr)-Deficient Mice: A Model of Mild Hyperhomocysteinemia.Investigative ophthalmology & visual science · 2015Article
- Induction of Neuronal Morphology in the 661W Cone Photoreceptor Cell Line with Staurosporine.PloS one · 2015Article
- RGC-5 cells.Investigative ophthalmology & visual science · 2013Article
- Alterations of retinal vasculature in cystathionine-Beta-synthase mutant mice, a model of hyperhomocysteinemia.Investigative ophthalmology & visual science · 2013Article
- Staurosporine-induced differentiation of the RGC-5 cell line leads to apoptosis and cell death at the lowest differentiating concentration.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2012Article
- Age-related changes in visual function in cystathionine-beta-synthase mutant mice, a model of hyperhomocysteinemia.Experimental eye research · 2012Article
- The role of N-methyl-D-aspartate receptor activation in homocysteine-induced death of retinal ganglion cells.Investigative ophthalmology & visual science · 2011Article
- The Wnt signaling pathway protects retinal ganglion cell 5 (RGC-5) cells from elevated pressure.Cellular and molecular neurobiology · 2011Article
- Visible light may directly induce nuclear DNA damage triggering the death pathway in RGC-5 cells.Molecular vision · 2011Article
- Prediction of diabetic retinopathy: role of oxidative stress and relevance of apoptotic biomarkers.The EPMA journal · 2010Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
purposeHomocysteine is implicated in ganglion cell death associated with glaucoma. To understand mechanisms of homocysteine-induced cell death, we analyzed the sensitivity of the RGC-5 cell line, differentiated using staurosporine, to physiologically-relevant levels of the excitotoxic amino acid homocysteine.
methodsRGC-5 cells were differentiated 24 hr using 316 nM staurosporine and tested for expression of Thy 1.2 via immunodetection, RT-PCR, and immunoblotting. The sensitivity of staurosporine-differentiated RGC-5 cells to physiological levels of homocysteine (50, 100, 250 microM) and to high levels of homocysteine (1 mM), glutamate (1 mM), and oxidative stress (25 microM:10 mU/ml xanthine:xanthine oxidase) was assessed by TUNEL assay and by immunodetection of cleaved caspase-3. The sensitivity of undifferentiated RGC-5 cells to high (1, 5, and 10 mM) homocysteine was also examined.
resultsUndifferentiated RGC-5 cells express Thy 1.2 mRNA and protein. Staurosporine-differentiated RGC-5 cells extend neurite processes and express Thy 1.2 after 24 hr differentiation; they express NF-L after 1 and 3 days differentiation. Treatment of staurosporine -differentiated RGC-5 cells with 50, 100, or 250 microM homocysteine did not alter neurite processes nor induce cell death (detected by TUNEL and active caspase-3) to a level greater than that observed in the control (non-homocysteine-treated, staurosporine-differentiated) cells. The 1 mM dosage of homocysteine in staurosporine-differentiated RGC-5 cells also did not induce cell death above control levels, although 18 hr treatment of non-differentiated RGC-5 cells with 5 mM homocysteine decreased survival by 50%.
conclusionsRGC-5 cells differentiated for 24 hr with 316 nM staurosporine project robust neurite processes and are positive for ganglion cell markers consistent with a more neuronal phenotype than non-staurosporine-differentiated RGC-5 cells. However, concentrations of homocysteine known to induce ganglion cell death in vivo and in primary ganglion cells are not sufficient to induce death of RGC-5 cells, even when they are differentiated with staurosporine.
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