ArticleCurrent eye research2010
Inhibitory effects of arresten on bFGF-induced proliferation, migration, and matrix metalloproteinase-2 activation in mouse retinal endothelial cells.
Article in Current eye research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- The effects of inhibition and siRNA knockdown of collagen-binding integrins on human umbilical vein endothelial cell migration and tube formation.Scientific reports · 2022Article
- An update on PTEN modulators - a patent review.Expert opinion on therapeutic patents · 2019Review
- Nicotine increased VEGF and MMP2 levels in the rat eye and kidney.Environmental science and pollution research international · 2019Article
- Evaluation of collagen derived antiangiogenic factors and matrix metalloproteinases in anterior lens epithelial cells of pediatric eyes with persistent fetal vasculature.Indian journal of ophthalmology · 2019Article
- Models of retinal diseases and their applicability in drug discovery.Expert opinion on drug discovery · 2018Review
- Nanoparticle-mediated arresten gene inhibits neointimal formation of vein grafts: an experimental research.Journal of thoracic disease · 2016Article
- Molecular underpinnings of corneal angiogenesis: advances over the past decade.International journal of ophthalmology · 2016Review
- bFGF Promotes the Migration of Human Dermal Fibroblasts under Diabetic Conditions through Reactive Oxygen Species Production via the PI3K/Akt-Rac1- JNK Pathways.International journal of biological sciences · 2015Article
- Type IV collagen α1-chain noncollagenous domain blocks MMP-2 activation both in-vitro and in-vivo.Scientific reports · 2014Article
- Developments in purification methods for obtaining and evaluation of collagen derived endogenous angioinhibitors.Protein expression and purification · 2014Review
- Gas6 stimulates angiogenesis of human retinal endothelial cells and of zebrafish embryos via ERK1/2 signaling.PloS one · 2014Article
- Extracellular matrix modulates angiogenesis in physiological and pathological conditions.BioMed research international · 2014Review
- Inhibition of elastin peptide-mediated angiogenic signaling mechanism(s) in choroidal endothelial cells by the α6(IV)NC1 collagen fragment.Investigative ophthalmology & visual science · 2013Article
- Regulation of Tumor Angiogenesis and Choroidal Neovascularization by Endogenous Angioinhibitors.Journal of cancer science & therapy · 2013Article
- L-arginine mediated renaturation enhances yield of human, α6 Type IV collagen non-collagenous domain from bacterial inclusion bodies.Protein and peptide letters · 2012Article
- Hyaluronic acid promotes angiogenesis by inducing RHAMM-TGFβ receptor interaction via CD44-PKCδ.Molecules and cells · 2012Article
- Role of matrix metalloproteinase-2 and -9 in the development of diabetic retinopathy.Journal of ocular biology, diseases, and informatics · 2012Article
- Proteolytically Derived Endogenous Angioinhibitors Originating from the Extracellular Matrix.Pharmaceuticals (Basel, Switzerland) · 2011Article
- The α7-nicotinic acetylcholine receptor and MMP-2/-9 pathway mediate the proangiogenic effect of nicotine in human retinal endothelial cells.Investigative ophthalmology & visual science · 2011Article
- Validation of Different Systems for Tumstatin Expression and its in-vitro and iv-vivo Activities.Journal of cancer science & therapy · 2010Article
Corrections and comments
- Retraction · 2020-04-02Falsification/Fabrication of Image · Investigation by Company/Institution · Investigation by ORI · Misconduct - Official Investigation(s) and/or Finding(s) · Misconduct by Author · · Yakkanti Sudhakar has aka of Yakkanti Akul Sudhakar and Sudhakar Yakkanti; Sudhakar Yakkanti has ORI finding of misconduct; https://ori.hhs.gov/content/case-summary-yakkanti-sudhakar;
- Retracted
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
purposeThe potential role of arresten (alpha1(IV)NC1) as an endogenous angiogenesis inhibitor in the prevention of bFGF mediated retinal angiogenesis and regulation of matrix metalloproteinase-2 activation has not been explored.
methodsMouse retinal endothelial cells (MREC) were cultured on type IV collagen and treated with basic fibroblast growth factor (bFGF) alone or in the presence of arresten at concentrations ranging from 1 to 10 microg/ml. The proliferation of MRECs were evaluated using 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay, and bFGF stimulated endothelial cell migration was assessed using Boyden chamber. Expression of matrix metalloproteinase-2 (MMP-2) was assessed by reverse transcription polymerase chain reaction (RT-PCR) analysis using RNA isolated from MRECs. Secretion and activation of MMP-2 in arresten-treated conditioned MREC growth medium was determined by gelatin zymography and Western blotting.
resultsDifferent doses of bFGF induced MREC proliferation was significantly inhibited upon arresten treatment (P < 0.005). The bFGF-induced migration was significantly inhibited by arresten at 1 and 10 microg/ml concentrations (P < 0.01). The bFGF stimulated expression of MMP-2 mRNA and secretion of MMP-2 in MREC was not affected and interestingly activation of MMP-2 was suppressed by arresten in a dose and time dependent manner.
conclusionsInhibitory effects of arresten on proliferation, migration and MMP-2 activation but not on expression and secretion of MMP-2 in MREC; this early work with arresten supports potential therapeutic action in retinal neovascularization dependent disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.