ArticleJournal of cellular physiology2010
Kinetic properties of ASC protein aggregation in epithelial cells.
Article in Journal of cellular physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 57 citations in OpenAlex.
- FACS-based detection of extracellular ASC specks from NLRP3 inflammasomes in inflammatory diseases.Clinical and experimental immunology · 2025Article
- Nanoscale organization of the endogenous ASC speck.iScience · 2023Article
- What are NLRP3-ASC specks? an experimental progress of 22 years of inflammasome research.Frontiers in immunology · 2023Review
- Mechanics of a molecular mousetrap-nucleation-limited innate immune signaling.Biophysical journal · 2021Review
- The ASC Speck and NLRP3 Inflammasome Function Are Spatially and Temporally Distinct.Frontiers in immunology · 2021Article
- Xi Lei San Attenuates Dextran Sulfate Sodium-Induced Colitis in Rats and TNF-Mediators of inflammation · 2021Article
- Quantifying Nucleation In Vivo Reveals the Physical Basis of Prion-like Phase Behavior.Molecular cell · 2018Article
- NLRC4 inflammasomopathies.Current opinion in allergy and clinical immunology · 2017Review
- Dynamics of in vivo ASC speck formation.The Journal of cell biology · 2017Article
- Assembly and regulation of ASC specks.Cellular and molecular life sciences : CMLS · 2017Review
- Mesenchymal Stem Cells Secretory Responses: Senescence Messaging Secretome and Immunomodulation Perspective.Frontiers in genetics · 2017Review
- ASC filament formation serves as a signal amplification mechanism for inflammasomes.Nature communications · 2016Article
- The cell biology of inflammasomes: Mechanisms of inflammasome activation and regulation.The Journal of cell biology · 2016Review
- Uncovering an Important Role for YopJ in the Inhibition of Caspase-1 in Activated Macrophages and Promoting Yersinia pseudotuberculosis Virulence.Infection and immunity · 2016Article
- Structural mechanisms of inflammasome assembly.The FEBS journal · 2015Review
- Artificial Loading of ASC Specks with Cytosolic Antigens.PloS one · 2015Article
- Structural mechanisms in NLR inflammasome signaling.Current opinion in structural biology · 2014Review
- Epigenetic regulation of ASC/TMS1 expression: potential role in apoptosis and inflammasome function.Cellular and molecular life sciences : CMLS · 2014Review
- Article
- The CARD plays a critical role in ASC foci formation and inflammasome signalling.The Biochemical journal · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Apoptosis-associated speck-like protein with CARD domain (ASC), an adaptor protein composed of caspase recruitment and pyrin domains, can efficiently self-associate to form a large spherical structure, called a speck. Although ASC aggregation is generally involved with both inflammatory processes and apoptosis, the detailed dynamics of speck formation have not been characterized. In this report, speck formation in HeLa cells transfected with ASC is examined by time-lapse live-imaging by confocal laser scanning microscopy. The results show that ASC aggregation is a very rapid and tightly regulated process. Prior to speck formation, soluble ASC aggregation is a low probability event, and the affinity of ASC subunits for one another is very low. Following a speck nucleation event, the affinity for further addition of ASC subunits increases dramatically, and aggregation is a highly energetically favorable reaction (Gibbs free energy approximately -40 kJ/mol). This leads to a rapid depletion of soluble ASC, making it highly unlikely that a second speck will form inside the same cell and assuring that speck formation is "all or none," with a well-defined end point. Comparison with kinetic models of the aggregation process indicates diffusion, instead of active transport, is the dominant process for speck growth. Though speck formation and aggresome formation share some properties, we show that the two processes are distinct.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.