ArticlePloS one2009
Nanoscale imaging of whole cells using a liquid enclosure and a scanning transmission electron microscope.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 99 citations in OpenAlex.
- Advancing Cryo-EM and Cryo-ET through Innovation in Sample Carriers: A Perspective.Analytical chemistry · 2025Review
- Imaging of Hydrated and Living Cells in Transmission Electron Microscope: Summary, Challenges, and Perspectives.ACS nano · 2025Review
- Article
- Liquid electron microscopy: then, now and future.Applied microscopy · 2019Review
- In situ graphene liquid cell-transmission electron microscopy study of insulin secretion in pancreatic islet cells.International journal of nanomedicine · 2019Article
- Nanometer Resolution Elemental Mapping in Graphene-Based TEM Liquid Cells.Nano letters · 2018Article
- Cell culture on hydrophilicity-controlled silicon nitride surfaces.World journal of microbiology & biotechnology · 2015Article
- A Structural Study of Escherichia coli Cells Using an In Situ Liquid Chamber TEM Technology.Journal of analytical methods in chemistry · 2015Article
- Correlative electron and fluorescence microscopy of magnetotactic bacteria in liquid: toward in vivo imaging.Scientific reports · 2014Article
- Dynamics of soft nanomaterials captured by transmission electron microscopy in liquid water.Journal of the American Chemical Society · 2014Article
- Enabling direct nanoscale observations of biological reactions with dynamic TEM.Microscopy (Oxford, England) · 2013Review
- Imaging interactions of metal oxide nanoparticles with macrophage cells by ultra-high resolution scanning electron microscopy techniques.Integrative biology : quantitative biosciences from nano to macro · 2012Article
- Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy.Micron (Oxford, England : 1993) · 2012Article
- Direct in situ observation of nanoparticle synthesis in a liquid crystal surfactant template.ACS nano · 2012Article
- Electron microscopy of specimens in liquid.Nature nanotechnology · 2011Review
- Controlled growth of nanoparticles from solution with in situ liquid transmission electron microscopy.Nano letters · 2011Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Nanoscale imaging techniques are needed to investigate cellular function at the level of individual proteins and to study the interaction of nanomaterials with biological systems. We imaged whole fixed cells in liquid state with a scanning transmission electron microscope (STEM) using a micrometer-sized liquid enclosure with electron transparent windows providing a wet specimen environment. Wet-STEM images were obtained of fixed E. coli bacteria labeled with gold nanoparticles attached to surface membrane proteins. Mammalian cells (COS7) were incubated with gold-tagged epidermal growth factor and fixed. STEM imaging of these cells resulted in a resolution of 3 nm for the gold nanoparticles. The wet-STEM method has several advantages over conventional imaging techniques. Most important is the capability to image whole fixed cells in a wet environment with nanometer resolution, which can be used, e.g., to map individual protein distributions in/on whole cells. The sample preparation is compatible with that used for fluorescent microscopy on fixed cells for experiments involving nanoparticles. Thirdly, the system is rather simple and involves only minimal new equipment in an electron microscopy (EM) laboratory.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.