ArticleMolecular vision2009
Protective effect of clusterin on oxidative stress-induced cell death of human corneal endothelial cells.
Article in Molecular vision, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- Clusterin, other extracellular chaperones, and eye disease.Progress in retinal and eye research · 2022Review
- Dexmedetomidine suppresses bupivacaine-induced parthanatos in human SH-SY5Y cells via the miR-7-5p/PARP1 axis-mediated ROS.Naunyn-Schmiedeberg's archives of pharmacology · 2021Article
- Cigarette Smoke Triggers Loss of Corneal Endothelial Cells and Disruption of Descemet's Membrane Proteins in Mice.Investigative ophthalmology & visual science · 2021Article
- Pathobiological Mechanisms of Endothelial Dysfunction Induced byInternational journal of medical sciences · 2020Article
- Apolipoprotein-J blocks increased cell injury elicited by ox-LDL via inhibiting ROS-CaMKII pathway.Lipids in health and disease · 2019Article
- Development of aqueous-based multi-herbal combination using principal component analysis and its functional significance in HepG2 cells.BMC complementary and alternative medicine · 2019Article
- Molecular Mechanisms and Genetics of Oxidative Stress in Alzheimer's Disease.Journal of Alzheimer's disease : JAD · 2019Review
- Growth Factors, Oxidative Damage, and Inflammation in Exfoliation Syndrome.Journal of glaucoma · 2018Review
- Acute toxicity profiling of the ethyl acetate fraction ofSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society · 2017Article
- Effect of cobalt(II) chloride hexahydrate on some human cancer cell lines.SpringerPlus · 2016Article
- Se-methylselenocysteine inhibits apoptosis induced by clusterin knockdown in neuroblastoma N2a and SH-SY5Y cell lines.International journal of molecular sciences · 2014Article
- Article
- Extracellular superoxide dismutase overexpression can reverse the course of hypoxia-induced pulmonary hypertension.Molecular medicine (Cambridge, Mass.) · 2012Article
- Clusterin protects H9c2 cardiomyocytes from oxidative stress-induced apoptosis via Akt/GSK-3β signaling pathway.Experimental & molecular medicine · 2011Article
- Cysteamine suppresses human peripheral blood mononuclear cells--human corneal endothelial cell reaction via reactive oxygen species reduction.Molecular vision · 2011Article
- Alterations in the aqueous humor proteome in patients with Fuchs endothelial corneal dystrophy.Molecular vision · 2010Article
- Expression of metabolic, tissue remodeling, oxidative stress, and inflammatory pathways in mammary tissue during involution in lactating dairy cows.Bioinformatics and biology insights · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the protective effect of clusterin on oxidative stress-induced cell death of human corneal endothelial cells.
methodsHuman corneal endothelial cells (HCECs) were cultured according to previously published methods. With treatment of various concentrations (0-50 mM) of tert-butyl hydroperoxide (tBHP) or clusterin, reactive oxygen species (ROS) producrion was measured using an oxidationsensitive fluorescent probe and 2'7'-dichlorofluorescin diacetate (DCFH-DA). Cell viability was assayed with a Cell Counting Kit-8.
resultsIn HCECs, DCF-DA staining revealed that cells treated with a higher concentration of tBHP had higher fluorescent intensity than cells treated with clusterin, compared to control cells. Clusterin significantly inhibited tBHP-induced ROS production. Cell viability decreased with higher tBHP concentration. Cells treated with clusterin had higher viability than control cells at 5 mM tBHP. Clusterin effectively protected HCECs from ROS-induced cell death.
conclusionsOur data suggest that clusterin may protect HCECs from oxidative injury-mediated cell death via inhibition of ROS production.
Indexed as
Identifiers
20019877PMC2793897W2097697539What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.