ArticleThe EMBO journal2010
Rapid reprogramming of haemoglobin structure-function exposes multiple dual-antimicrobial potencies.
Article in The EMBO journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Whole blood exchange ameliorates acute hemolytic anemia by reducing inflammation and oxidative stress in rats.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Hemoglobin induces inflammation through NF-kB signaling pathway and causes cell oxidative damage in grass carp (Frontiers in immunology · 2022Article
- The Antibacterial Activity of Erythrocytes From GooseFrontiers in immunology · 2021Article
- Insights into the Natural Defenses of a Coral Reef Fish Against Gill Ectoparasites: Integrated Metabolome and Microbiome Approach.Metabolites · 2020Article
- The public's risk perception of blood transfusion in Saudi Arabia.Journal of blood medicine · 2018Article
- Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin.Cellular and molecular life sciences : CMLS · 2017Review
- Expression of hemoglobin-α and β subunits in human vaginal epithelial cells and their functional significance.PloS one · 2017Article
- Macrophages and Iron Metabolism.Immunity · 2016Review
- Ixodes scapularis Tick Saliva Proteins Sequentially Secreted Every 24 h during Blood Feeding.PLoS neglected tropical diseases · 2016Article
- The Iron age of host-microbe interactions.EMBO reports · 2015Review
- Response of Neutrophils to Extracellular Haemoglobin and LTA in Human Blood System.EBioMedicine · 2015Article
- HbAHP-25, an In-Silico Designed Peptide, Inhibits HIV-1 Entry by Blocking gp120 Binding to CD4 Receptor.PloS one · 2015Article
- Hemoglobin expression in nonerythroid cells: novel or ubiquitous?International journal of inflammation · 2014Review
- Preferential silent survival of intracellular bacteria in hemoglobin-primed macrophages.Journal of innate immunity · 2014Article
- A perspective on the role of extracellular hemoglobin on the innate immune system.DNA and cell biology · 2013Review
- Menaquinone biosynthesis potentiates haem toxicity in Staphylococcus aureus.Molecular microbiology · 2012Article
- Lipid-free apolipoprotein A-I exerts an antioxidative role against cell-free hemoglobin.American journal of clinical and experimental immunology · 2012Article
- Effect of Rabbit Epididymal Antimicrobial Peptide, REHbβP, on LPS-Induced Proinflammatory Cytokine Responses in Human Vaginal Cells In Vitro.International journal of peptides · 2012Article
- Delineation of lipopolysaccharide (LPS)-binding sites on hemoglobin: from in silico predictions to biophysical characterization.The Journal of biological chemistry · 2011Article
- A rabbit vaginal cell-derived antimicrobial peptide, RVFHbαP, blocks lipopolysaccharide-mediated inflammation in human vaginal cells in vitro.Clinical and vaccine immunology : CVI · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The intrinsic cytotoxicity of cell-free haemoglobin (Hb) has hampered the development of reliable Hb-based blood substitutes for over seven decades. Notably, recent evidence shows that the Hb deploys this cytotoxic attack against invading microbes, albeit, through an unknown mechanism. Here, we unraveled a rapid molecular reprogramming of the Hb structure-function triggered by virulent haemolytic pathogens that feed on the haem-iron. On direct contact with the microbe, the Hb unveils its latent antimicrobial potency, where multiple antimicrobial fragments are released, each harbouring coordinated 'dual-action centres': microbe binding and pseudoperoxidase (POX) cycle activity. The activated Hb fragments anchor onto the microbe while the juxtaposed POX instantly unleashes a localized oxidative shock, killing the pathogen-in-proximity. This concurrent action conceivably restricts the diffusion of free radicals. Furthermore, the host astutely protects itself from self-cytotoxicity by simultaneously releasing endogenous antioxidants. We found that this decryption mechanism of antimicrobial potency is conserved in the ancient invertebrate respiratory protein, indicating its fundamental significance. Our definition of dual-antimicrobial centres in the Hb provides vital clues for designing a safer Hb-based oxygen carrier blood substitute.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.