ArticleInvestigative ophthalmology & visual science2010
Formation and disassembly of adherens and tight junctions in the corneal endothelium: regulation by actomyosin contraction.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Characterization of the Functional Transient Receptor Potential Vanilloid Channels 1 to 4 in Human Corneal Endothelial Cells.Investigative ophthalmology & visual science · 2026Article
- Exploring corneal endothelial cell dynamics: structure, functions and therapeutic approaches.Molecular biology reports · 2025Review
- Proliferator-Activated Receptor Alpha Inhibits Abnormal Extracellular Matrix Accumulation and Maintains Energy Metabolism in Late-Onset Fuchs Endothelial Corneal Dystrophy.Investigative ophthalmology & visual science · 2025Article
- Topical Ocular Drug Delivery: The Impact of Permeation Enhancers.Pharmaceutics · 2025Review
- New Perspective on Aqueous Humor Circulation: Retina Takes the Lead.International journal of molecular sciences · 2025Review
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- Corneal Endothelial-like Cells Derived from Induced Pluripotent Stem Cells for Cell Therapy.International journal of molecular sciences · 2023Review
- TGF-β-Mediated Modulation of Cell-Cell Interactions in Postconfluent Maturing Corneal Endothelial Cells.Investigative ophthalmology & visual science · 2022Article
- Article
- Transcriptomic analysis of differential gene expression during chick periocular neural crest differentiation into corneal cells.Developmental dynamics : an official publication of the American Association of Anatomists · 2019Article
- TGF-β1 promotes cell barrier function upon maturation of corneal endothelial cells.Scientific reports · 2018Article
- Biomechanical relationships between the corneal endothelium and Descemet's membrane.Experimental eye research · 2016Review
- Pitfalls in assessing microvascular endothelial barrier function: impedance-based devices versus the classic macromolecular tracer assay.Scientific reports · 2016Article
- Phenotypes of trypsin- and collagenase-prepared bovine corneal endothelial cells in the presence of a selective Rho kinase inhibitor, Y-27632.Molecular vision · 2015Article
- The role of epithelial tight junctions involved in pathogen infections.Molecular biology reports · 2014Review
- The Hippo pathway: regulators and regulations.Genes & development · 2013Review
- Molecular mechanisms underlying the corneal endothelial pump.Experimental eye research · 2012Review
- Cell signaling in regulation of the barrier integrity of the corneal endothelium.Experimental eye research · 2012Review
- P2Y receptors as regulators of lung endothelial barrier integrity.Journal of cardiovascular disease research · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Purpose. To determine the role of actin cytoskeleton in the disassembly and reformation of adherens junctions (AJs) and tight junctions (TJs) in bovine corneal endothelial monolayers. Methods. Disassembly and reformation of AJs and TJs were induced by extracellular Ca(2+) depletion and subsequent add-back of Ca(2+), respectively. Resultant changes in the transendothelial electrical resistance (TER), an indicator of integrity of TJs, were measured based on electrical cell-substrate impedance. Phosphorylated myosin light chain (ppMLC), a biochemical measure of actomyosin contraction, and activation of its upstream regulatory molecule RhoA-GTP were assessed by Western blot analysis. Results. Extracellular Ca(2+) depletion led to activation of RhoA, increase in ppMLC, decrease in TER, contraction of the perijunctional actomyosin ring (PAMR), and redistribution of zonula occludens-1 (ZO-1) and cadherins. These effects were reversed on Ca(2+) add-back. Pretreatment with Y-27632 and blebbistatin (as inhibitors of actomyosin contraction) reduced the rate of decline in TER, opposed the contraction of the PAMR, and blocked the redistribution of ZO-1 and cadherins. Both drugs reduced the recovery in TER and opposed the normal redistribution of ZO-1 and cadherins on Ca(2+) add-back. Cytochalasin D, which led to dissolution of the PAMR, also reduced the recovery of TER on Ca(2+) add-back. Conclusions. The (Ca(2+) depletion)-induced disassembly of AJs accelerates the breakdown of TJs through a concomitant increase in the actomyosin contraction of the PAMR. However, these data on reassembly show that a contractile tone of the PAMR is essential for assembly of the apical junctional complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.