ArticleInvestigative ophthalmology & visual science2010
Role of the Fas pathway in Pseudomonas aeruginosa keratitis.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 26 citations in OpenAlex.
- FAS mediates apoptosis, inflammation, and treatment of pathogen infection.Frontiers in cellular and infection microbiology · 2025Review
- Ocular surface immune transcriptome and tear cytokines in corneal infection patients.Frontiers in cellular and infection microbiology · 2024Article
- A novel Fas-binding outer membrane protein and lipopolysaccharide of Leptospira interrogans induce macrophage apoptosis through the Fas/FasL-caspase-8/-3 pathway.Emerging microbes & infections · 2018Article
- Ocular diseases: immunological and molecular mechanisms.International journal of ophthalmology · 2016Review
- Pattern recognition receptors in microbial keratitis.Eye (London, England) · 2015Review
- Emodin ameliorates lipopolysaccharides-induced corneal inflammation in rats.International journal of ophthalmology · 2015Article
- Interleukin 17 regulates Mer tyrosine kinase-positive cells in Pseudomonas aeruginosa keratitis.Investigative ophthalmology & visual science · 2014Article
- Cholecystokinin octapeptide antagonizes apoptosis in human retinal pigment epithelial cells.Neural regeneration research · 2014Article
- Glutaredoxin-1 attenuates S-glutathionylation of the death receptor fas and decreases resolution of Pseudomonas aeruginosa pneumonia.American journal of respiratory and critical care medicine · 2014Article
- Increased resistance to Staphylococcus aureus endophthalmitis in BALB/c mice: Fas ligand is required for resolution of inflammation but not for bacterial clearance.Infection and immunity · 2013Article
- TREM-2 promotes host resistance against Pseudomonas aeruginosa infection by suppressing corneal inflammation via a PI3K/Akt signaling pathway.Investigative ophthalmology & visual science · 2013Article
- Lithium chloride promotes host resistance against Pseudomonas aeruginosa keratitis.Molecular vision · 2013Article
- HIF-1α is essential for effective PMN bacterial killing, antimicrobial peptide production and apoptosis in Pseudomonas aeruginosa keratitis.PLoS pathogens · 2013Article
- Article
- Puerarin antagonizes peroxyntrite-induced injury in retinal pigment epithelial cells.Neural regeneration research · 2012Article
- Escherichia coli induces apoptosis in human monocytic U937 cells through the Fas/FasL signaling pathway.Molecular and cellular biochemistry · 2011Article
- Control of peroxyntrite-induced production of inducible nitric oxide synthase isoforms and antagonism of cholecystokinin octapeptide -8 in retinal pigment epithelial cells in vivo.International journal of ophthalmology · 2011Article
- Peroxynitrite-induced expression of inducible nitric oxide synthase and activated apoptosis via nuclear factor-kappa B pathway in retinal pigment epithelial cells and antagonism of cholecystokinin octapeptide-8 in vitro.International journal of ophthalmology · 2011Article
- Animal models of bacterial keratitis.Journal of biomedicine & biotechnology · 2011Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe role of the Fas pathway was tested in Pseudomonas aeruginosa-infected mouse cornea by contrasting the responses of FasL(-/-) and wild-type (WT) mice.
methodsTUNEL staining, real-time RT-PCR, immunostaining, and ELISA assay were used.
resultsCompared with WT (resistant) mice, BALB/c FasL(-/-) exhibited significantly elevated bacterial counts and polymorphonuclear leukocyte numbers at 1 and 3 days postinfection (p.i.) and worse outcomes from disease. Similar bacterial challenges in C57BL/6 FasL(-/-) compared with WT mice also led to worsened disease as evidenced by earlier corneal perforation in the susceptible mouse strain. Intense TUNEL staining of apoptotic cells was seen earlier (1 day vs. 3 days) p.i. in BALB/c WT than in knockout mice, This earlier apoptotic pattern correlated with increased expression of caspases 3, 8, and 9 and BAX and with decreased expression of the antiapoptotic molecule Bcl-2. Furthermore, expression levels of the proinflammatory molecule TNF-alpha and its receptor, MIP-2, inducible nitric oxide synthase (iNOS), and nitrite also were significantly elevated in the infected cornea of BALB/c FasL(-/-) compared with WT mice. In vitro, LPS-stimulated Mphi from BALB/c FasL(-/-) mice expressed significantly less caspase 3 and 9, BAX, and IL-10 and more TNF-alpha, MIP-2, and IL-1beta than did cells from WT mice.
conclusionsFas-FasL interaction in the cornea balances the host innate immune response to improve disease outcome by promoting earlier apoptosis and regulating proinflammatory cytokines/chemokines and nitric oxide (nitrite) production. Dysregulation of this interaction contributes to bystander tissue damage, enhancing nutrients for bacterial growth and worsened disease outcome after P. aeruginosa infection.
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