ArticleProceedings of the National Academy of Sciences of the United States of America2010
Chloroplast acetyl-CoA carboxylase activity is 2-oxoglutarate-regulated by interaction of PII with the biotin carboxyl carrier subunit.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 175 citations in OpenAlex.
- Lipid MRI in plant science: principles and potential areas of application.Journal of experimental botany · 2026Review
- Amino Acids as Multifunctional Molecules in Plants: From Fundamental Metabolism to Precision Agriculture.Plants (Basel, Switzerland) · 2026Review
- Understanding the dynamic nature of plant lipid anabolic and catabolic metabolism is key to sustainable oilseed engineering.The New phytologist · 2026Review
- PII controls ACCase assembly to co-regulate oil and protein metabolism in plants.Plant physiology · 2026Article
- PII interactions with the acetyl-CoA carboxylase subunits BADC and BCCP co-regulate lipid and nitrogen metabolism in Arabidopsis.Plant physiology · 2025Article
- Article
- REGULATOR OF FATTY ACID SYNTHESIS proteins regulate de novo fatty acid synthesis by modulating hetACCase distribution.The Plant cell · 2024Article
- Evolution of the regulatory subunits for the heteromeric acetyl-CoA carboxylase.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- The PII protein interacts with the Amt ammonium transport and modulates nitrate/nitrite assimilation in mycobacteria.Frontiers in microbiology · 2024Article
- Protein interactomes for plant lipid biosynthesis and their biotechnological applications.Plant biotechnology journal · 2023Review
- Comparative genomics reveals a unique nitrogen-carbon balance system in Asteraceae.Nature communications · 2023Article
- Fus3, as a Critical Kinase in MAPK Cascade, Regulates Aflatoxin Biosynthesis by Controlling the Substrate Supply in Aspergillus flavus, Rather than the Cluster Genes Modulation.Microbiology spectrum · 2022Article
- Article
- Downregulation of the E2 Subunit of 2-Oxoglutarate Dehydrogenase Modulates Plant Growth by Impacting Carbon-Nitrogen Metabolism in Arabidopsis thaliana.Plant & cell physiology · 2021Article
- Dissection of the Mechanisms of Growth Inhibition Resulting from Loss of the PII Protein in the Cyanobacterium Synechococcus elongatus PCC 7942.Plant & cell physiology · 2021Article
- The Classical, Yet Controversial, First Enzyme of Lipid Synthesis: Escherichia coli Acetyl-CoA Carboxylase.Microbiology and molecular biology reviews : MMBR · 2021Review
- Discrimination and Quantitation of Biologically Relevant Carboxylate Anions Using A [Dye•PAMAM] Complex.Sensors (Basel, Switzerland) · 2021Article
- Biotin attachment domain-containing proteins mediate hydroxy fatty acid-dependent inhibition of acetyl CoA carboxylase.Plant physiology · 2021Article
- Increasing the Ascomycin Yield by Relieving the Inhibition of Acetyl/Propionyl-CoA Carboxylase by the Signal Transduction Protein GlnB.Frontiers in microbiology · 2021Article
- The Role of Sugar Signaling in Regulating Plant Fatty Acid Synthesis.Frontiers in plant science · 2021Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The PII protein is a signal integrator involved in the regulation of nitrogen metabolism in bacteria and plants. Upon sensing of cellular carbon and energy availability, PII conveys the signal by interacting with target proteins, thereby modulating their biological activity. Plant PII is located to plastids; therefore, to identify new PII target proteins, PII-affinity chromatography of soluble extracts from Arabidopsis leaf chloroplasts was performed. Several proteins were retained only when Mg-ATP was present in the binding medium and they were specifically released from the resin by application of a 2-oxoglutarate-containing elution buffer. Mass spectroscopy of SDS/PAGE-resolved protein bands identified the biotin carboxyl carrier protein subunits of the plastidial acetyl-CoA carboxylase (ACCase) and three other proteins containing a similar biotin/lipoyl-binding motif as putative PII targets. ACCase is a key enzyme initiating the synthesis of fatty acids in plastids. In in vitro reconstituted assays supplemented with exogenous ATP, recombinant Arabidopsis PII inhibited chloroplastic ACCase activity, and this was completely reversed in the presence of 2-oxoglutarate, pyruvate, or oxaloacetate. The inhibitory effect was PII-dose-dependent and appeared to be PII-specific because ACCase activity was not altered in the presence of other tested proteins. PII decreased the V(max) of the ACCase reaction without altering the K(m) for acetyl-CoA. These data show that PII function has evolved between bacterial and plant systems to control the carbon metabolism pathway of fatty acid synthesis in plastids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.