ArticleJournal of the American Chemical Society2010
Non-heme iron oxygenases generate natural structural diversity in carbapenem antibiotics.
Article in Journal of the American Chemical Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Evolution of Methods for the Study of Cobalamin-Dependent Radical SAM Enzymes.ACS bio & med chem Au · 2022Article
- Article
- Roles of 2-oxoglutarate oxygenases and isopenicillin N synthase in β-lactam biosynthesis.Natural product reports · 2018Review
- Mechanism of the C5 stereoinversion reaction in the biosynthesis of carbapenem antibiotics.Science (New York, N.Y.) · 2014Article
- Identification and characterization of the carbapenem MM 4550 and its gene cluster in Streptomyces argenteolus ATCC 11009.Chembiochem : a European journal of chemical biology · 2014Article
- Mechanistic insights into the bifunctional non-heme iron oxygenase carbapenem synthase by active site saturation mutagenesis.Journal of the American Chemical Society · 2013Article
- Autoproteolytic activation of ThnT results in structural reorganization necessary for substrate binding and catalysis.Journal of molecular biology · 2012Article
- Axial and equatorial ligand effects on biomimetic cysteine dioxygenase model complexes.Organic & biomolecular chemistry · 2012Article
- A high-throughput screen for the engineered production of β-lactam antibiotics.ACS chemical biology · 2012Article
- Engineering the synthetic potential of β-lactam synthetase and the importance of catalytic loop dynamics.MedChemComm · 2012Article
- Definition of the common and divergent steps in carbapenem β-lactam antibiotic biosynthesis.Chembiochem : a European journal of chemical biology · 2011Article
- Mutational analysis of the thienamycin biosynthetic gene cluster from Streptomyces cattleya.Antimicrobial agents and chemotherapy · 2011Article
- Diverting non-haem iron catalysed aliphatic C-H hydroxylations towards desaturations.Nature chemistry · 2011Article
- New insights into the formation of fungal aromatic polyketides.Nature reviews. Microbiology · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Carbapenems are a clinically important antibiotic family. More than 50 naturally occurring carbapenam/ems are known and are distinguished primarily by their C-2/C-6 side chains where many are only differentiated by the oxidation states of these substituents. With a limited palette of variations the carbapenem family comprises a natural combinatorial library, and C-2/C-6 oxidation is associated with increased efficacy. We demonstrate that ThnG and ThnQ encoded by the thienamycin gene cluster in Streptomyces cattleya oxidize the C-2 and C-6 moieties of carbapenems, respectively. ThnQ stereospecifically hydroxylates PS-5 (5) giving N-acetyl thienamycin (2). ThnG catalyzes sequential desaturation and sulfoxidation of PS-5 (5), giving PS-7 (7) and its sulfoxide (9). The enzymes are relatively substrate selective but are proposed to give rise to the oxidative diversity of carbapenems produced by S. cattleya, and orthologues likely function similarly in allied streptomyces. Elucidating the roles of ThnG and ThnQ will focus further investigations of carbapenem antibiotic biosynthesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.