ArticleJournal of virology2010
Inhibition of sterol biosynthesis reduces tombusvirus replication in yeast and plants.
Article in Journal of virology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 90 citations in OpenAlex.
- Screening bacterial effectors and human virus proteins in yeast to identify host factors driving tombusvirus RNA recombination: a role for autophagy and membrane phospholipid content.Journal of virology · 2025Article
- Reprogrammed Plant Metabolism During Viral Infections: Mechanisms, Pathways and Implications.Molecular plant pathology · 2025Review
- Proviral role of ATG2 autophagy related protein in tomato bushy stunt virus replication through bulk phospholipid transfer into the viral replication organelle.Molecular biology of the cell · 2024Article
- Subversion of selective autophagy for the biogenesis of tombusvirus replication organelles inhibits autophagy.PLoS pathogens · 2024Article
- Down-regulation of tomato STEROL GLYCOSYLTRANSFERASE 1 perturbs plant development and facilitates viroid infection.Journal of experimental botany · 2023Article
- Phytosterol metabolism in plant positive-strand RNA virus replication.Plant cell reports · 2022Review
- Inhibition of SC4MOL and HSD17B7 shifts cellular sterol composition and promotes oligodendrocyte formation.RSC chemical biology · 2022Article
- Sugarcane mosaic virus orchestrates the lactate fermentation pathway to support its successful infection.Frontiers in plant science · 2022Article
- An airway organoid-based screen identifies a role for the HIF1α-glycolysis axis in SARS-CoV-2 infection.Cell reports · 2021Article
- Tombusviruses Target a Major Crossroad in the Endocytic and Recycling Pathways via Co-opting Rab7 Small GTPase.Journal of virology · 2021Article
- Dynamic interplay between the co-opted Fis1 mitochondrial fission protein and membrane contact site proteins in supporting tombusvirus replication.PLoS pathogens · 2021Article
- The retromer is co-opted to deliver lipid enzymes for the biogenesis of lipid-enriched tombusviral replication organelles.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Reconstitution of an RNA Virus Replicase in Artificial Giant Unilamellar Vesicles Supports Full Replication and Provides Protection for the Double-Stranded RNA Replication Intermediate.Journal of virology · 2020Article
- Co-opted Cellular Sac1 Lipid Phosphatase and PI(4)P Phosphoinositide Are Key Host Factors during the Biogenesis of the Tombusvirus Replication Compartment.Journal of virology · 2020Article
- Targeting host lipid flows: Exploring new antiviral and antibiotic strategies.Cellular microbiology · 2019Review
- Metabolism and Biological Activities of 4-Methyl-Sterols.Molecules (Basel, Switzerland) · 2019Review
- Host Lipids in Positive-Strand RNA Virus Genome Replication.Frontiers in microbiology · 2019Review
- Shaping the flavivirus replication complex: It is curvaceous!Cellular microbiology · 2018Review
- Chloroplast: the Trojan horse in plant-virus interaction.Molecular plant pathology · 2018Review
- Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Plant Virus Replication.Frontiers in plant science · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The replication of plus-strand RNA viruses depends on subcellular membranes. Recent genome-wide screens have revealed that the sterol biosynthesis genes ERG25 and ERG4 affected the replication of Tomato bushy stunt virus (TBSV) in a yeast model host. To further our understanding of the role of sterols in TBSV replication, we demonstrate that the downregulation of ERG25 or the inhibition of the activity of Erg25p with an inhibitor (6-amino-2-n-pentylthiobenzothiazole; APB) leads to a 3- to 5-fold reduction in TBSV replication in yeast. In addition, the sterol biosynthesis inhibitor lovastatin reduced TBSV replication by 4-fold, confirming the importance of sterols in viral replication. We also show reduced stability for the p92(pol) viral replication protein as well as a decrease in the in vitro activity of the tombusvirus replicase when isolated from APB-treated yeast. Moreover, APB treatment inhibits TBSV RNA accumulation in plant protoplasts and in Nicotiana benthamiana leaves. The inhibitory effect of APB on TBSV replication can be complemented by exogenous stigmasterol, the main plant sterol, suggesting that sterols are required for TBSV replication. The silencing of SMO1 and SMO2 genes, which are orthologs of ERG25, in N. benthamiana reduced TBSV RNA accumulation but had a lesser inhibitory effect on the unrelated Tobacco mosaic virus, suggesting that various viruses show different levels of dependence on sterol biosynthesis for their replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.