ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2010
Kinetics of the UV-induced DNA damage response in relation to cell cycle phase. Correlation with DNA replication.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 54 citations in OpenAlex.
- Five dominant amino acid substitution signatures shape tumour immunity.Molecular systems biology · 2026Article
- BTG3-dependent VCP/p97 nuclear translocation is required for efficient repair of UV-induced DNA lesions.Nucleic acids research · 2025Article
- Effects of Exosomes From Hypoxia-Induced Adipose-Derived Stem Cells on Ameliorating Photoaging.Clinical, cosmetic and investigational dermatology · 2025Article
- Inflammaging: Expansion of Molecular Phenotype and Role in Age-Associated Female Infertility.Biomedicines · 2024Article
- Synthesis and Antiproliferative Insights of Lipophilic Ru(II)-Hydroxy Stearic Acid Hybrid Species.Molecules (Basel, Switzerland) · 2023Article
- γH2AX in the S Phase after UV Irradiation Corresponds to DNA Replication and Does Not Report on the Extent of DNA Damage.Molecular and cellular biology · 2020Article
- Sulfur Mustard Analog Mechlorethamine (Bis(2-chloroethyl)methylamine) Modulates Cell Cycle Progression via the DNA Damage Response in Human Lung Epithelial A549 Cells.Chemical research in toxicology · 2019Article
- Influence of a pre-stimulation with chronic low-dose UVB on stress response mechanisms in human skin fibroblasts.PloS one · 2017Article
- Nucleotide Excision Repair and Vitamin D--Relevance for Skin Cancer Therapy.International journal of molecular sciences · 2016Review
- Oxidative Stress and Carbonyl Lesions in Ulcerative Colitis and Associated Colorectal Cancer.Oxidative medicine and cellular longevity · 2016Review
- Review
- Expression of the p12 subunit of human DNA polymerase δ (Pol δ), CDK inhibitor p21(WAF1), Cdt1, cyclin A, PCNA and Ki-67 in relation to DNA replication in individual cells.Cell cycle (Georgetown, Tex.) · 2014Article
- New insights into cell cycle and DNA damage response machineries through high-resolution AMICO quantitative imaging cytometry.Cell proliferation · 2013Article
- The PIM-2 kinase is an essential component of the ultraviolet damage response that acts upstream to E2F-1 and ATM.The Journal of biological chemistry · 2013Article
- Laser scanning cytometry: principles and applications-an update.Methods in molecular biology (Clifton, N.J.) · 2013Review
- Quantitative profiling of DNA damage and apoptotic pathways in UV damaged cells using PTMScan Direct.International journal of molecular sciences · 2012Article
- Multispectral imaging flow cytometry reveals distinct frequencies of γ-H2AX foci induction in DNA double strand break repair defective human cell lines.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2012Article
- Relationship of DNA damage signaling to DNA replication following treatment with DNA topoisomerase inhibitors camptothecin/topotecan, mitoxantrone, or etoposide.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2012Article
- Induction of DNA damage signaling by oxidative stress in relation to DNA replication as detected using "click chemistry".Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2011Article
- Functional relevance of the histone gammaH2Ax in the response to DNA damaging agents.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
It has been reported that exposure to UV light triggers DNA damage response (DDR) seen as induction of gammaH2AX not only in S- but also in G(1)-phase cells. In the present study, in addition to gammaH2AX, we assessed other markers of DDR, namely phosphorylation of ATM on Ser1981, of ATM/ATR substrate on Ser/Thr at SQ/TQ cluster domains and of the tumor suppressor p53 on Ser15, in human pulmonary carcinoma A549 cells irradiated with 50 J/m(2) of UV-B. Phosphorylation of these proteins detected with phospho-specific Abs and measured by laser scanning cytometry in relation the cell cycle phase was found to be selective to S-phase cells. The kinetics of phosphorylation of ATM was strikingly similar to that of ATM/ATR substrate, peaking at 30 min after UV irradiation and followed by rapid dephosphorylation. The peak of H2AX phosphorylation was seen at 2 h and the peak of p53 phosphorylation at 4 h after exposure to UV light. Local high spatial density of these phospho-proteins reported by intensity of maximal pixel of immunofluorescence in the DDR nuclear foci was distinctly more pronounced in the early compared to late portion of S-phase. Exposure of cells to UV following 1 h pulse-labeling of their DNA with 5-ethynyl-2'deoxyuridine (EdU) made it possible to correlate the extent of DNA replication during the pulse with the extent of the UV-induced H2AX phosphorylation within the same cells. This correlation was very strong (R(2) = 0.98) and the cells that did not incorporate EdU showed no evidence of H2AX phosphorylation. The data are consistent with the mechanism in which stalling of DNA replication forks upon collision with the primary UV-induced DNA lesions and likely formation of double-strand DNA breaks triggers DDR. The prior reports (including our own) on induction of gammaH2AX in G(1) cells by UV may have erroneously identified cells initiating DNA replication following UV exposure as G(1) cells due to the fact that their DNA content did not significantly differ from that of G(1) cells that had not initiated DNA replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.