ArticlePlant cell reports2010
Rapid in planta evaluation of root expressed transgenes in chimeric soybean plants.
Article in Plant cell reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 57 citations in OpenAlex.
- Identification of genes governing resistance to PCN (Globodera rostochiensis) through transcriptome analysis in Solanum tuberosum.Functional & integrative genomics · 2023Article
- CONSTANS-LIKE 1a positively regulates salt and drought tolerance in soybean.Plant physiology · 2023Article
- Amenability of anGM crops & food · 2022Article
- Purslane (Portulaca oleracea L.) as a novel green-bioreactor for expression of human serum albumin (HSA) gene.Transgenic research · 2022Article
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- Cyst nematode bio-communication with plants: implications for novel management approaches.Pest management science · 2021Review
- Host-derived gene silencing of parasite fitness genes improves resistance to soybean cyst nematodes in stable transgenic soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- RNAi-Based Biocontrol of Wheat Nematodes Using Natural Poly-Component Biostimulants.Frontiers in plant science · 2019Article
- From model to crop: functional characterization of SPL8 in M. truncatula led to genetic improvement of biomass yield and abiotic stress tolerance in alfalfa.Plant biotechnology journal · 2018Article
- An improved method forPlant methods · 2018Article
- RNA Interference: A Novel Source of Resistance to Combat Plant Parasitic Nematodes.Frontiers in plant science · 2017Review
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- Identification and functional analysis of secreted effectors from phytoparasitic nematodes.BMC microbiology · 2016Review
- A high efficient protocol for soybean root transformation by Agrobacterium rhizogenes and most stable reference genes for RT-qPCR analysis.Plant cell reports · 2015Article
- Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production.Plant cell reports · 2015Article
- Expression of a cystatin transgene can confer resistance to root lesion nematodes in Lilium longiflorum cv. 'Nellie White'.Transgenic research · 2015Article
- A comparison study of Agrobacterium-mediated transformation methods for root-specific promoter analysis in soybean.Plant cell reports · 2014Article
- The status of RNAi-based transgenic research in plant nematology.Frontiers in microbiology · 2014Review
- Analysis of tomato gene promoters activated in syncytia induced in tomato and potato hairy roots by Globodera rostochiensis.Transgenic research · 2013Article
- Knocking-down Meloidogyne incognita proteases by plant-delivered dsRNA has negative pleiotropic effect on nematode vigor.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Production of stable transgenic plants is one factor that limits rapid evaluation of tissue specific transgene expression. To hasten the assessment of transgenes in planta, we evaluated the use of chimeric soybean seedlings expressing transgenic products in roots. Tap roots from four-day old seedlings (cultivars 'Jack' and KS4704) were excised and hairy roots were induced from hypocotyls via Agrobacterium rhizogenes-mediated transformation. Inoculated hypocotyls were screened on a MS-based medium containing either 200 mg/L kanamycin or 20 mg/L hygromycin. Beta-glucuronidase (GUS) activity assay indicated that highest GUS expression was observed in hypocotyls exposed to a 4-d pre-inoculation time, a neutral pH (7.0) for the co-cultivation medium. A 170-bp of the Fib-1 gene and 292-bp of the Y25C1A.5 gene fragments, both related to nematode reproduction and fitness, were cloned independently into pANDA35HK vector using a Gateway cloning strategy. The resulting RNAi constructs of the genes fragments were transformed into soybean using the chimeric hairy root system and evaluated for its effect on soybean cyst nematode (Heterodera glycines) fecundity. Confirmation of transformation was attained by polymerase chain reaction and Southern-blot analysis, and some potential for suppression of H. glycines reproduction was detected for the two constructs. This method takes on average four weeks to produce chimeric plants ready for transgene analysis.
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