ArticleJournal of gastroenterology2010
Comparison of HCV-associated gene expression and cell signaling pathways in cells with or without HCV replicon and in replicon-cured cells.
Article in Journal of gastroenterology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it.
- An integrated transcriptomic and meta-analysis of hepatoma cells reveals factors that influence susceptibility to HCV infection.PloS one · 2011Pooled it
- Article
- MicroRNA Profile of HCV Spontaneous Clarified Individuals, Denotes Previous HCV Infection.Journal of clinical medicine · 2019Article
- Opposite Effects of Two Human ATG10 Isoforms on Replication of a HCV Sub-genomic Replicon Are Mediated via Regulating Autophagy Flux in Zebrafish.Frontiers in cellular and infection microbiology · 2018Article
- Metabolic alterations and hepatitis C: From bench to bedside.World journal of gastroenterology · 2016Review
- Article
- HCV NS5A Up-Regulates COX-2 Expression via IL-8-Mediated Activation of the ERK/JNK MAPK Pathway.PloS one · 2015Article
- Psammaplin A inhibits hepatitis C virus NS3 helicase.Journal of natural medicines · 2013Article
- CD133: a stem cell biomarker and beyond.Experimental hematology & oncology · 2013Article
- Inhibitory effects of caffeic acid phenethyl ester derivatives on replication of hepatitis C virus.PloS one · 2013Article
- HCV IRES-mediated core expression in zebrafish.PloS one · 2013Article
- Comparison of Helicobacter bilis-Associated Protein Expression in Huh7 Cells Harbouring HCV Replicon and in Replicon-Cured Cells.International journal of hepatology · 2012Article
- PPARs and HCV-Related Hepatocarcinoma: A Mitochondrial Point of View.PPAR research · 2012Article
- Article
- Alteration of hepatic nuclear receptor-mediated signaling pathways in hepatitis C virus patients with and without a history of alcohol drinking.Hepatology (Baltimore, Md.) · 2011Article
- The interaction between HCV and nuclear receptor-mediated pathways.Pharmacology & therapeutics · 2011Review
- Analysis of interferon signaling by infectious hepatitis C virus clones with substitutions of core amino acids 70 and 91.Journal of virology · 2011Article
- Article
- Inhibition of hepatitis C virus replication by a specific inhibitor of serine-arginine-rich protein kinase.Antimicrobial agents and chemotherapy · 2010Article
- Gene expression profiling indicates the roles of host oxidative stress, apoptosis, lipid metabolism, and intracellular transport genes in the replication of hepatitis C virus.Journal of virology · 2010Article
Corrections and comments
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Authors and funding
21 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHepatitis C virus (HCV) replication is affected by several host factors. Here, we screened host genes and molecular pathways that are involved in HCV replication by comprehensive analyses using two genotypes of HCV replicon-expressing cells, their cured cells and naïve Huh7 cells.
methodsHuh7 cell lines that stably expressed HCV genotype 1b or 2a replicon were used. The cured cells were established by treating HCV replicon cells with interferon-alpha. Expression of 54,675 cellular genes was analyzed by GeneChip DNA microarray. The data were analyzed by using the KEGG Pathway database.
resultsHierarchical clustering analysis showed that the gene-expression profiles of each cell group constituted clear clusters of naïve, HCV replicon-expressed, and cured cell lines. The pathway process analysis between the replicon-expressing and the cured cell lines identified significantly altered pathways, including MAPK, steroid biosynthesis and TGF-beta signaling pathways, suggesting that these pathways were affected directly by HCV replication. Comparison of cured and naïve Huh7 cells identified pathways, including steroid biosynthesis and sphingolipid metabolism, suggesting that these pathways were required for efficient HCV replication. Cytoplasmic lipid droplets were obviously increased in replicon-expressing and cured cells as compared to naïve cells. HCV replication was significantly suppressed by peroxisome proliferator-activated receptor (PPAR)-alpha agonists but augmented by PPAR-gamma agonists.
conclusionComprehensive gene expression and pathway analyses show that lipid biosynthesis pathways are crucial to support proficient virus replication. These metabolic pathways could constitute novel antiviral targets against HCV.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.